Walden W E, Thach R E
Biochemistry. 1986 Apr 22;25(8):2033-41. doi: 10.1021/bi00356a030.
The translation of a small number of mRNAs in mouse SC-1 fibroblasts can be stimulated by cycloheximide, under conditions where the synthesis of most proteins is inhibited. These mRNAs are ordinarily present in small polyribosomes or messenger ribonucleoprotein particles, although the addition of cycloheximide drives them into large (greater than or equal to 5) polysomes. These mRNAs cannot be translated in vitro unless they are extracted with phenol. With such treatment, however, they are translated with normal competitive efficiencies. In iron-poor media, the mRNA for ferritin exhibits several of the distinctive kinetic properties of this class of mRNAs. With iron supplementation, however, ferritin translation appears normal. These observations are consistent with the existence of translational induction/repression systems in eukaryotes. Several types of evidence suggest that repressors may act by interfering with the interaction between mRNAs and limiting translational initiation components.
在大多数蛋白质合成受到抑制的条件下，环己酰亚胺可刺激小鼠SC - 1成纤维细胞中少数mRNA的翻译。这些mRNA通常存在于小多核糖体或信使核糖核蛋白颗粒中，不过加入环己酰亚胺会促使它们进入大的（大于或等于5）多核糖体中。除非用苯酚提取，否则这些mRNA无法在体外进行翻译。然而，经过这种处理后，它们能以正常的竞争效率进行翻译。在缺铁培养基中，铁蛋白的mRNA表现出这类mRNA的几种独特动力学特性。然而，补充铁后，铁蛋白的翻译似乎正常。这些观察结果与真核生物中存在翻译诱导/抑制系统相一致。几种证据表明，阻遏物可能通过干扰mRNA与有限的翻译起始成分之间的相互作用来发挥作用。
