Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal.
CIIMAR/CIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, Portugal.
Int J Syst Evol Microbiol. 2023 Apr;73(4). doi: 10.1099/ijsem.0.005850.
Organisms with distinctive biological features and cellular organization constitute the bacterial phylum . In this study, we formally describe a novel isolate, strain ICT_H6.2, isolated from sediment samples collected in the brackish environment of the Tagus River estuary (Portugal) using an iChip-based culturing technique. The 16S rRNA gene analysis placed this strain into the phylum and family , with a similarity value of 98.0 % to its closest relative Pan181, the currently only known member of the genus. Strain ICT_H6.2 has a genome size of 7.8 Mbp and a DNA G+C content of 59.6 mol %. Strain ICT_H6.2 is heterotrophic, aerobic and capable of microaerobic growth. This strain grows from 10 to 37 °C and from pH 6.5 to 10.0, requires salt to grow, and can tolerate up to 4 % (w/v) NaCl. Diverse nitrogen and carbon sources are utilized for growth. Morphologically, strain ICT_H6.2 is white to beige pigmented, spherical to ovoid in shape and around 1.4×1.1 µm in size. The strain clusters mainly in aggregates and younger cells show motility. Ultrastructural studies showed a cell plan with cytoplasmatic membrane invaginations and unusual filamentous structures with hexagonal organization in transversal section. Morphological, physiological and genomic comparison between strain ICT_H6.2 and its closest relatives strongly suggests it represents a novel species within the genus , for which we propose the name sp. nov., represented by strain ICT_H6.2 as the type strain (=CECT 30574=DSM 114064).
具有独特生物特征和细胞组织的生物构成了细菌门。在本研究中，我们使用基于 iChip 的培养技术，正式描述了一种从葡萄牙塔古斯河口咸水环境中采集的沉积物样本中分离出来的新型分离株 ICT_H6.2。16S rRNA 基因分析将该菌株归入 和科，与最接近的亲缘物种 Pan181 的相似度为 98.0 %，而 Pan181 是目前已知的唯一属成员。菌株 ICT_H6.2 的基因组大小为 7.8 Mbp，DNA G+C 含量为 59.6 mol %。菌株 ICT_H6.2 是异养、需氧和微需氧生长的。该菌株在 10 到 37°C 之间、pH 值为 6.5 到 10.0 之间生长，需要盐分才能生长，并能耐受高达 4 %（w/v）的 NaCl。多种氮源和碳源可用于生长。形态上，菌株 ICT_H6.2 呈白色到米色色素沉着，球形到卵形，大小约为 1.4×1.1 µm。该菌株主要聚集在聚集体中，年轻的细胞表现出运动性。超微结构研究显示，细胞平面具有细胞质膜内陷和异常的丝状结构，在横截面上呈六边形组织。ICT_H6.2 菌株与其最接近的亲缘关系的形态、生理和基因组比较强烈表明，它代表了属内的一个新物种，我们建议将其命名为 sp. nov.，以 ICT_H6.2 菌株为模式菌株（=CECT 30574=DSM 114064）。
