Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007, Porto, Portugal.
CIMAR/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros de Leixões, Av. General Norton de Matos s/n, 4450-208, Matosinhos, Portugal.
Antonie Van Leeuwenhoek. 2023 Nov;116(11):1209-1225. doi: 10.1007/s10482-023-01877-2. Epub 2023 Sep 22.
A bacterial strain was isolated from a brackish water sample of Tagus river, Alcochete, Portugal and was designated TO1_6. It forms light pink colonies on M13 medium supplemented with N-acetylglucosamine. Cells are pear-shaped to spherical, form rosettes and divide by budding. Strain TO1_6 presents a mesophilic and neutrophilic profile, with optimum growth at 20 to 25 °C and pH 7.0 to 7.5, and vitamin supplementation is not required to promote its growth. The genome of the novel isolate is 7.77 Mbp in size and has a DNA G + C content of 56.3%. Based on its 16S rRNA gene sequence, this strain is affiliated with the phylum Planctomycetota. Further taxonomic characterization using additional phylogenetic markers, namely rpoB gene sequence (encoding the β-subunit of the DNA-dependent RNA polymerase), as well as Percentage of conserved proteins, average nucleotide identity and average amino acid identity, suggest the affiliation of strain TO1_6 to the genus Stieleria, a recently described taxon in the family Pirellulaceae, order Pirellulales and class Planctomycetia. Based on the genotypic, phylogenetic and physiological characterization, we here describe a new species represented by the type strain TO1_6 (= CECT 30432, = LMG 32465), for which the name Stieleria tagensis sp. nov. is proposed.
从葡萄牙塔古斯河的一个咸水样本中分离到一株细菌菌株，命名为 TO1_6。它在补充 N-乙酰葡萄糖胺的 M13 培养基上形成浅粉色菌落。细胞呈梨形至球形，形成玫瑰花结并通过出芽分裂。菌株 TO1_6 呈现出嗜温和嗜中性的特性，最佳生长温度为 20 至 25°C，pH 值为 7.0 至 7.5，不需要维生素补充来促进其生长。该新分离株的基因组大小为 7.77 Mbp，DNA G+C 含量为 56.3%。根据其 16S rRNA 基因序列，该菌株属于浮霉菌门。使用其他系统发育标记物，即 rpoB 基因序列（编码 DNA 依赖性 RNA 聚合酶的β亚基）以及保守蛋白百分比、平均核苷酸同一性和平均氨基酸同一性，进一步进行分类特征描述，表明菌株 TO1_6 属于 Stieleria 属，这是一个最近在 Pirellulaceae 科、Pirellulales 目和 Planctomycetia 纲中描述的分类群。基于基因型、系统发育和生理特征，我们在此描述了一个以模式菌株 TO1_6（=CECT 30432，=LMG 32465）为代表的新物种，建议将其命名为 Stieleria tagensis sp. nov.。
