Centro de Investigación Biomédica (CENBIO), Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170527, Ecuador.
The University of Queensland, Institute for Molecular Bioscience, 306 Carmody Road, St Lucia, Australia.
Trends Plant Sci. 2023 Aug;28(8):955-967. doi: 10.1016/j.tplants.2023.03.013. Epub 2023 Apr 18.
Microalgal chloroplasts, such as those of the model organism Chlamydomonas reinhardtii, are emerging as a new platform to produce recombinant proteins, including industrial enzymes, diagnostics, as well as animal and human therapeutics. Improving transgene expression and final recombinant protein yields, at laboratory and industrial scales, require optimization of both environmental and cellular factors. Most studies on C. reinhardtii have focused on optimization of cellular factors. Here, we review the regulatory influences of environmental factors, including light (cycle time, intensity, and quality), carbon source (CO and organic), and temperature. In particular, we summarize their influence via the redox state, cis-elements, and trans-factors on biomass and recombinant protein production to support the advancement of emerging large-scale light-driven biotechnology applications.
微藻叶绿体，如模式生物莱茵衣藻（Chlamydomonas reinhardtii）的叶绿体，正逐渐成为生产重组蛋白的新平台，包括工业酶、诊断试剂以及动物和人类治疗药物。提高转基因表达和最终重组蛋白产量，无论是在实验室规模还是工业规模，都需要优化环境和细胞因素。大多数关于莱茵衣藻的研究都集中在细胞因素的优化上。在这里，我们综述了环境因素的调控影响，包括光（周期时间、强度和质量）、碳源（CO 和有机）和温度。特别地，我们总结了它们通过氧化还原状态、顺式元件和反式因子对生物量和重组蛋白生产的影响，以支持新兴的大规模光驱动生物技术应用的发展。
