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头孢地尼对金黄色葡萄球菌的抗菌活性，并与红霉素和泰利霉素进行比较。

The antimicrobial activity of cethromycin against Staphylococcus aureus and compared with erythromycin and telithromycin.

机构信息

Department of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Shenzhen Nanshan People's Hospital and the 6Th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, 518052, China.

Department of Critical Care Medicine and the Key Lab of Endogenous Infection, Shenzhen Nanshan People's Hospital and the 6Th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, 518052, China.

出版信息

BMC Microbiol. 2023 Apr 20;23(1):109. doi: 10.1186/s12866-023-02858-1.

DOI:10.1186/s12866-023-02858-1

PMID:37081393

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10116812/

Abstract

BACKGROUND

This study aims to explore the antibacterial activity of cethromycin against Staphylococcus aureus (S. aureus), and its relationship with multilocus sequence typing (MLST), erythromycin ribosomal methylase (erm) genes and macrolide-lincosamide-streptogramin B (MLSB) phenotypes of S. aureus.

RESULTS

The minimum inhibitory concentrations (MICs) of cethromycin against 245 S. aureus clinical isolates ranged from 0.03125 to ≥ 8 mg/L, with the resistance of 38.8% in 121 methicillin-resistant S. aureus (MRSA). This study also found that cethromycin had strong antibacterial activity against S. aureus, with the MIC ≤ 0.5 mg/L in 55.4% of MRSA and 60.5% of methicillin-sensitive S. aureus (MSSA), respectively. The main MLSTs of 121 MRSA were ST239 and ST59, and the resistance of ST239 isolates to cethromycin was higher than that in ST59 isolates (P = 0.034). The top five MLSTs of 124 MSSA were ST7, ST59, ST398, ST88 and ST120, but there was no difference in the resistance of MSSA to cethromycin between these STs. The resistance of ermA isolates to cethromycin was higher than that of ermB or ermC isolates in MRSA (P = 0.016 and 0.041, respectively), but the resistance of ermB or ermC isolates to cethromycin was higher than that of ermA isolates in MSSA (P = 0.019 and 0.026, respectively). The resistance of constitutive MLSB (cMLSB) phenotype isolates to cethromycin was higher than that of inducible MLSB (iMLSB) phenotype isolates in MRSA (P < 0.001) or MSSA (P = 0.036). The ermA, ermB and ermC genes was mainly found in ST239, ST59 and ST1 isolates in MRSA, respectively. Among the MSSA, the ermC gene was more detected in ST7, ST88 and ST120 isolates, but more ermB genes were detected in ST59 and ST398 isolates. The cMLSB phenotype was more common in ST239 and ST59 isolates of MRSA, and was more frequently detected in ST59, ST398, and ST120 isolates of MSSA.

CONCLUSION

Cethromycin had strong antibacterial activity against S. aureus. The resistance of MRSA to cethromycin may had some clonal aggregation in ST239. The resistance of S. aureus carrying various erm genes or MLSB phenotypes to cethromycin was different.

摘要

背景

本研究旨在探讨头孢地美酯对金黄色葡萄球菌（S. aureus）的抗菌活性及其与多位点序列分型（MLST）、红霉素核糖体甲基化酶（erm）基因和金黄色葡萄球菌大环内酯-林可酰胺-链阳菌素 B（MLSB）表型的关系。

结果

头孢地美酯对 245 株金黄色葡萄球菌临床分离株的最低抑菌浓度（MIC）范围为 0.03125 至≥8mg/L，其中 121 株耐甲氧西林金黄色葡萄球菌（MRSA）的耐药率为 38.8%。本研究还发现，头孢地美酯对金黄色葡萄球菌具有很强的抗菌活性，其中 55.4%的 MRSA 和 60.5%的甲氧西林敏感金黄色葡萄球菌（MSSA）的 MIC≤0.5mg/L。121 株 MRSA 的主要 MLST 型为 ST239 和 ST59，ST239 分离株对头孢地美酯的耐药性高于 ST59 分离株（P=0.034）。124 株 MSSA 的前 5 种 MLST 型为 ST7、ST59、ST398、ST88 和 ST120，但这些 ST 型之间 MSSA 对头孢地美酯的耐药性无差异。MRSA 中 ermA 分离株对头孢地美酯的耐药性高于 ermB 或 ermC 分离株（P=0.016 和 0.041），而 MSSA 中 ermB 或 ermC 分离株对头孢地美酯的耐药性高于 ermA 分离株（P=0.019 和 0.026）。MRSA 中固有 MLSB（cMLSB）表型分离株对头孢地美酯的耐药性高于诱导性 MLSB（iMLSB）表型分离株（P<0.001）或 MSSA（P=0.036）。ermA、ermB 和 ermC 基因主要存在于 MRSA 的 ST239、ST59 和 ST1 分离株中。在 MSSA 中，ermC 基因更多地存在于 ST7、ST88 和 ST120 分离株中，而 ermB 基因更多地存在于 ST59 和 ST398 分离株中。cMLSB 表型在 MRSA 的 ST239 和 ST59 分离株中更为常见，在 MSSA 的 ST59、ST398 和 ST120 分离株中更为常见。