Turbant Florian, Hamoui Omar El, Partouche David, Sandt Christophe, Busi Florent, Wien Frank, Arluison Véronique
Laboratoire Léon Brillouin LLB, CEA, CNRS UMR12, Université Paris Saclay, CEA Saclay, 91191 Gif-sur-Yvette, France.
Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland.
BBA Adv. 2021 Oct 30;1:100029. doi: 10.1016/j.bbadva.2021.100029. eCollection 2021.
Nucleic acid amyloid proteins interactions have been observed in the past few years. These interactions often promote protein aggregation. Nevertheless, molecular basis and physiological consequences of these interactions are still poorly understood. Additionally, it is unknown whether the nucleic acid promotes the formation of self-assembly due to direct interactions or indirectly sequences surrounding the amyloid region. Here we focus our attention on a bacterial amyloid, Hfq. This protein is a pleiotropic bacterial regulator that mediates many aspects of nucleic acids metabolism. The protein notably mediates mRNA stability and translation efficiency by using stress-related small non coding regulatory RNA. In addition, Hfq, thanks to its amyloid C-terminal region, binds and compacts DNA. A combination of experimental methodologies, including synchrotron radiation circular dichroism (SRCD), gel shift assay and infrared (FTIR) spectroscopy have been used to probe the interaction of Hfq C-terminal region with DNA. We clearly identify important amino acids in this region involved in DNA binding and polymerization properties. This allows to understand better how this bacterial amyloid interacts with DNA. Possible functional consequence to answer to stresses are discussed.
在过去几年中已观察到核酸与淀粉样蛋白之间的相互作用。这些相互作用常常促进蛋白质聚集。然而，这些相互作用的分子基础和生理后果仍知之甚少。此外，尚不清楚核酸是由于直接相互作用还是通过淀粉样区域周围的间接序列来促进自组装的形成。在这里，我们将注意力集中在一种细菌淀粉样蛋白Hfq上。这种蛋白质是一种多效性细菌调节剂，介导核酸代谢的许多方面。该蛋白质通过利用与应激相关的小非编码调节RNA，显著介导mRNA稳定性和翻译效率。此外，Hfq由于其淀粉样C末端区域，能够结合并压缩DNA。包括同步辐射圆二色性（SRCD）、凝胶迁移实验和红外（FTIR）光谱在内的多种实验方法已被用于探究Hfq C末端区域与DNA的相互作用。我们明确鉴定出该区域中参与DNA结合和聚合特性的重要氨基酸。这有助于更好地理解这种细菌淀粉样蛋白如何与DNA相互作用。文中还讨论了应对压力可能产生的功能后果。
