Laboratoire Léon Brillouin LLB, CEA, CNRS UMR12, Université Paris Saclay, Gif-sur-Yvette, France.
Université de Paris, Paris, France.
Methods Mol Biol. 2020;2113:135-148. doi: 10.1007/978-1-0716-0278-2_11.
Circular dichroism (CD) spectroscopy is a fast and simple technique providing important information about the conformation of nucleic acids, proteins, sugars, lipids, and their interactions between each other. This electronic absorption spectroscopy method is extremely sensitive to any change in molecular structure containing asymmetric molecules. While numerous reviews describe how to analyze deoxyribonucleic acid (DNA) structures using CD, analyses of ribonucleic acids (RNAs) are scarce. Nevertheless, RNAs are important molecules involved in a multitude of roles in the cell. In this chapter, we present applications of synchrotron radiation circular dichroism (SRCD) extending the spectral range down to 170 nm, improving structural analysis of RNA, including the analysis of helical parameters and alternative structures found in RNA. The effects of temperature to measure thermodynamic parameters and analyze ribonucleoprotein complexes will also be presented.
圆二色性（CD）光谱是一种快速而简单的技术，提供有关核酸、蛋白质、糖、脂质及其相互作用构象的重要信息。这种电子吸收光谱法对含有不对称分子的任何分子结构变化都非常敏感。虽然有许多评论描述了如何使用 CD 分析脱氧核糖核酸（DNA）结构，但对核糖核酸（RNA）的分析却很少。然而，RNA 是参与细胞中多种功能的重要分子。在本章中，我们介绍了同步辐射圆二色性（SRCD）的应用，将光谱范围扩展到 170nm，改善了 RNA 的结构分析，包括对 RNA 中螺旋参数和替代结构的分析。还将介绍温度对测量热力学参数和分析核糖核蛋白复合物的影响。
