Shrivastava Prabhanshu, Gosavi Suchitra, Ghatge Dipak, Naik Aishwarya, Marlapalle Archana, Krishna Abhinandh
Departments of Oral Pathology, Government Dental College and Hospital, Nagpur, Maharashtra, India.
J Oral Maxillofac Pathol. 2022 Oct-Dec;26(4):518-523. doi: 10.4103/jomfp.jomfp_236_22. Epub 2022 Dec 22.
The study indicated that XPD and XPG gene polymorphism is associated with the development of oral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma. Xeroderma pigmentosa (XP) is a part of the complex DNA repair system. Xeroderma pigmentosum group G (XPG) and xeroderma pigmentosum group D (XPD) gene function in the nucleotide excision repair (NER) pathway. XPG and/or XPD gene alterations can cause defective DNA repair efficiency which ultimately leads to genomic instability and carcinogenesis. Thus, this study helps in early detection of OPMDs among individuals who have not yet developed any oral lesions and also helps in the management of oral squamous cell carcinoma as if XP gene polymorphism is known.
The aim of the study was to evaluate the expression of XPD and XPG gene polymorphism in oral squamous cell carcinoma cases. The study also had the objective to evaluate and compare the expression of XPG and XPD gene polymorphism in oral squamous cell carcinoma (OSCC) cases, tobacco chewers without any oral lesions, and normal healthy individuals without any habit.
A total of 150 subjects were included in the study and genotyped for the expression of XPD (AC) and XPG (GC) gene polymorphism using polymerase chain reaction (PCR) and agarose gel electrophoresis method.
XPD genotype for the study shows that most of the cases of OSCC show heterozygous (AC) genotype (64%), whereas in tobacco chewers without any oral lesions wild (AA) genotype (54%) is more common than other types. XPG genotype for the study shows that wild (GG) type is the most dominant genotype both in OSCC cases (78%) and tobacco chewers without any oral lesion (56%).
The study shows the association of XPD and XPG gene polymorphism with the risk of developing OPMDs and oral cancer.
该研究表明，XPD和XPG基因多态性与口腔潜在恶性疾病（OPMDs）及口腔鳞状细胞癌的发生有关。着色性干皮病（XP）是复杂DNA修复系统的一部分。着色性干皮病G组（XPG）和着色性干皮病D组（XPD）基因在核苷酸切除修复（NER）途径中发挥作用。XPG和/或XPD基因改变可导致DNA修复效率缺陷，最终导致基因组不稳定和致癌。因此，本研究有助于在尚未出现任何口腔病变的个体中早期检测OPMDs，并且在已知XP基因多态性的情况下，也有助于口腔鳞状细胞癌的管理。
本研究的目的是评估XPD和XPG基因多态性在口腔鳞状细胞癌病例中的表达。该研究还旨在评估和比较XPG和XPD基因多态性在口腔鳞状细胞癌（OSCC）病例、无任何口腔病变的嚼烟者以及无任何习惯的正常健康个体中的表达。
本研究共纳入150名受试者，使用聚合酶链反应（PCR）和琼脂糖凝胶电泳法对XPD（AC）和XPG（GC）基因多态性的表达进行基因分型。
该研究的XPD基因型显示，大多数OSCC病例表现为杂合子（AC）基因型（64%），而在无任何口腔病变的嚼烟者中，野生型（AA）基因型（54%）比其他类型更为常见。该研究的XPG基因型显示，野生型（GG）在OSCC病例（78%）和无任何口腔病变的嚼烟者（56%）中都是最主要的基因型。
该研究表明XPD和XPG基因多态性与发生OPMDs和口腔癌的风险相关。
