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评估香精油蒸汽对生物气溶胶中细菌数量的影响。

Evaluation of effects of essential oil vapors on the bacterial count in bioaerosols.

作者信息

Shetty Vishwaprakash, Varalakshmi K Sri, Prakash A Jacob, Lakshmi M Vijaya, Harsha M

机构信息

Department of Oral Pathology, Lenora Institute of Dental Sciences, Rajanagaram, Andhra Pradesh, India.

出版信息

J Oral Maxillofac Pathol. 2022 Oct-Dec;26(4):601. doi: 10.4103/jomfp.jomfp_279_21. Epub 2022 Dec 22.

Abstract

BACKGROUND

The aerosols generated during dental treatments contain bacteria and other microorganisms that penetrate the body through the respiratory system of dental surgeons and cause infectious diseases. Several studies have been done to reduce these hazards. The aim of the present study is to evaluate the effects of the plant extract essential oil (EO) vapors of Neem, Clove, Cinnamon bark, Thyme, Lemon Grass, and on the bacterial count in bioaerosols near dental units.

MATERIALS AND METHODS

Sampling was taken on nutrient blood agar plates by placing them open near dental units using passive air sampling method, before commencement of treatment for 1 h, during treatments for 2 h, and after introducing EO vapors for 2 h. The collected samples were taken for incubation at 37°C for 48 h. The colonies formed were counted in colony-forming units per cubic meter and taken for statistical analysis.

RESULTS

After comparing the obtained results, it was found that there was a significant reduction ( < 0.05) in the bacterial count for about 43% near the dental units after the introduction of the EO vapours.

CONCLUSION

It is concluded that natural extracts like EOs can reduce bacterial contamination near dental units in the vapourized state, thereby reducing the health hazards in Dental Health Professionals.

摘要

背景

牙科治疗过程中产生的气溶胶含有细菌和其他微生物,它们通过牙科外科医生的呼吸系统进入人体并引发传染病。已经开展了多项研究来降低这些危害。本研究的目的是评估印楝、丁香、肉桂树皮、百里香、柠檬草的植物提取物精油(EO)蒸汽对牙科设备附近生物气溶胶中细菌数量的影响。

材料与方法

采用被动空气采样法,在治疗开始前1小时、治疗期间2小时以及引入EO蒸汽2小时后,将营养血琼脂平板打开放置在牙科设备附近进行采样。采集的样本在37°C下培养48小时。对形成的菌落以每立方米菌落形成单位进行计数,并进行统计分析。

结果

比较所得结果后发现,引入EO蒸汽后,牙科设备附近的细菌数量显著减少(<0.05),降幅约为43%。

结论

得出的结论是,像EOs这样的天然提取物在汽化状态下可以减少牙科设备附近的细菌污染,从而降低牙科保健专业人员的健康风险。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c95a/10112105/d5e772a1dfc4/JOMFP-26-601-g001.jpg

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