2015 - 2021年中国食用动物分离株的血清型、抗菌药物耐药性及毒力的综合分析 - Suppr

Comprehensive profiling of serotypes, antimicrobial resistance and virulence of isolates from food animals in China, 2015-2021.

作者信息

Guo Lili, Xiao Tianan, Wu Liqin, Li Yan, Duan Xiaoxiao, Liu Wenhua, Liu Kaidi, Jin Wenjie, Ren Hao, Sun Jian, Liu Yahong, Liao Xiaoping, Zhao Yongda

机构信息

College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.

Qingdao Bolin Biotechnology Co., Qingdao, China.

出版信息

Front Microbiol. 2023 Apr 4;14:1133241. doi: 10.3389/fmicb.2023.1133241. eCollection 2023.

INTRODUCTION

is a ubiquitous foodborne pathogen and mainly transmitted to human farm-to-fork chain through contaminated foods of animal origin.

METHODS

In this study, we investigated the serotypes, antimicrobial resistance and virulence of from China.

RESULTS

A total of 617 isolates were collected from 4 major food animal species across 23 provi nces in China from 2015-2021. Highest prevalence were observed in Guangdong (44.4%) and Sandong (23.7%). Chickens (43.0%) was shown to be the major source of contamination, followed by pigs (34.5%) and ducks (18.5%). The number of increased significantly from 5.51% to 27.23% during 2015-2020. . Derby (17.3%), . Enteritidis (13.1%) and . Typhimurium (11.4%) were the most common serotypes among 41 serotypes identifiedin this study. Antibiotic susceptibility testing showing that the majority of the isolates were resistant to neomycin (99.7%), tetracycline (98.1%), ampicillin (97.4%), sulfadiazine/trimethoprim (97.1%), nalidixic acid (89.1%), doxycycline (83.1%), ceftria xone (70.3%), spectinomycin (67.7%), florfenicol (60.0%), cefotaxime (52.0%) and lomefloxacin (59.8%). The rates of resistance to multiple antibiotics in . Derby and .Typhimurium were higher than that in . Enteritidis. However, the rate of resistance to fosfomycin were observed from higher to lower by . Derby, . Enteritidis, and . Typhimurium. Biofilm formation ability analysis found that 88.49%of the Salmonella were able to produce biofilms, of which 236 Salmonella isolates were strong biofilm producer. Among the 26 types of antibiotics resistance genes (ARGs) were identified in this study, 4 ARGs (tetB,sul2,aadA2, and aph(3')-IIa) were highly prevalent. In addition, 5 β-lactam resistance genes ( , , , , and ) and 7 quinolone resistance genes (, , , , , , and ) were detected among these isolates. 12 out of 17 virulence genes selected in this study were commonly presented in the chromosomes of tested isolate, with a detection rate of over 80%, including , , , , , , , , , , , .

DISCUSSION

This study provided a systematical updating on surveillance on prevalence of from food animals in China, shedding the light on continued vigilance for in food animals.

引言

[病原体名称]是一种普遍存在的食源性病原体，主要通过受污染的动物源性食品在从农场到餐桌的链条中传播给人类。

方法

在本研究中，我们调查了来自中国的[病原体名称]的血清型、抗菌药物耐药性和毒力。

结果

2015年至2021年期间，从中国23个省份的4种主要食用动物物种中总共收集了617株[病原体名称]分离株。在广东（44.4%）和山东（23.7%）观察到最高的[病原体名称]流行率。鸡（43.0%）被证明是[病原体名称]污染的主要来源，其次是猪（34.5%）和鸭（18.5%）。2015年至2020年期间，[病原体名称]的数量从5.51%显著增加到27.23%。德比沙门氏菌（17.3%）、肠炎沙门氏菌（13.1%）和鼠伤寒沙门氏菌（11.4%）是本研究中鉴定出的41种血清型中最常见的血清型。抗生素敏感性测试表明，大多数[病原体名称]分离株对新霉素（99.7%）、四环素（98.1%）、氨苄青霉素（97.4%）、磺胺嘧啶/甲氧苄啶（97.1%）、萘啶酸（89.1%）、强力霉素（83.1%）、头孢曲松（70.3%）、壮观霉素（67.7%）、氟苯尼考（60.0%）、头孢噻肟（52.0%）和洛美沙星（59.8%）耐药。德比沙门氏菌和鼠伤寒沙门氏菌对多种抗生素的耐药率高于肠炎沙门氏菌。然而，观察到德比沙门氏菌、肠炎沙门氏菌和鼠伤寒沙门氏菌对磷霉素的耐药率从高到低。生物膜形成能力分析发现，88.49%的沙门氏菌能够产生生物膜，其中236株沙门氏菌分离株是强生物膜产生者。在本研究中鉴定出的26种抗生素抗性基因（ARGs）中，4种ARGs（tetB、sul2、aadA2和aph(3')-IIa）高度流行。此外，在这些分离株中检测到5种β-内酰胺抗性基因（[基因名称1]、[基因名称2]、[基因名称3]、[基因名称4]和[基因名称5]）和7种喹诺酮抗性基因（[基因名称6]、[基因名称7]、[基因名称8]、[基因名称9]、[基因名称10]、[基因名称11]和[基因名称12]）。在本研究中选择的17个毒力基因中有12个通常存在于测试分离株的染色体中，检出率超过80%，包括[基因名称13]、[基因名称14]、[基因名称15]、[基因名称16] [基因名称17]、[基因名称18]、[基因名称19]、[基因名称20]、[基因名称21]、[基因名称22]、[基因名称23]、[基因名称24]。