Jia Tony Z, Nishikawa Shota, Fujishima Kosuke
Earth-Life Science Institute, Tokyo Institute of Technology, 2-12-1-IE-1 Ookayama, Meguro-ku, Tokyo 152-8550, Japan.
Blue Marble Space Institute of Science, 600 1st Ave, Floor 1, Seattle, WA 98104, USA.
BBA Adv. 2022 Mar 5;2:100049. doi: 10.1016/j.bbadva.2022.100049. eCollection 2022.
One goal of origins of life research is to understand how primitive informational and catalytic biopolymers emerged and evolved. Recently, a number of sequencing techniques have been applied to analysis of replicating and evolving primitive biopolymer systems, providing a sequence-specific and high-resolution view of primitive chemical processes. Here, we review application of sequencing techniques to analysis of synthetic and primitive nucleic acids and polypeptides. This includes next-generation sequencing of primitive polymerization and evolution processes, followed by discussion of other novel biochemical techniques that could contribute to sequence analysis of primitive biopolymer driven chemical systems. Further application of sequencing to origins of life research, perhaps as a life detection technology, could provide insight into the origin and evolution of informational and catalytic biopolymers on early Earth or elsewhere.
生命起源研究的一个目标是了解原始信息和催化生物聚合物是如何出现和进化的。最近，一些测序技术已被应用于对复制和进化的原始生物聚合物系统的分析，从而提供了对原始化学过程的序列特异性和高分辨率视图。在这里，我们综述了测序技术在合成和原始核酸及多肽分析中的应用。这包括对原始聚合和进化过程的下一代测序，随后讨论其他可能有助于对原始生物聚合物驱动的化学系统进行序列分析的新型生化技术。测序技术在生命起源研究中的进一步应用，也许作为一种生命检测技术，可以深入了解早期地球或其他地方信息和催化生物聚合物的起源和进化。
