School of Biological and Chemical Engineering, Yangzhou Polytechnic College, Yangzhou, China.
Yangzhou Engineering Research Center of Agricultural Products Intelligent Measurement and Control & Cleaner Production, Yangzhou Polytechnic College, Yangzhou, China.
Anim Biotechnol. 2023 Dec;34(8):3681-3692. doi: 10.1080/10495398.2023.2200433. Epub 2023 Apr 21.
Colibacillosis is a complex disease that caused by avian pathogenic (APEC), resulting in huge economic loss to the global poultry industry and threatening to human health. Alternative splicing (AS) is a universal post-transcriptional regulatory mechanism, which can simultaneously produce many proteins from a single gene to involve in various diseases and individual development. Herein, we characterized genome-wide AS events in wild type macrophages (WT) and APEC infected macrophages (APEC) by high-throughput RNA sequencing technology. A total of 751 differentially expressed (DE) AS genes were identified in the comparison of APEC vs. WT, including 587 of SE, 114 of MXE, 25 of RI, 17 of A3 and 8 of A5 event. Functional analysis showed that these identified DE AS genes were involved in 'Endocytosis', 'p53 signaling pathway', 'MAPK signaling pathway', 'NOD-like receptor signaling pathway', 'Ubiquitin mediated proteolysis' and 'Focal adhesion' immune related pathways. In summary, we comprehensively investigate AS events during APEC infection. This study has expanded our understanding of the process of APEC infection and provided new insights for further treatment options for APEC infection.
大肠杆菌病是一种由禽致病性大肠杆菌（APEC）引起的复杂疾病，给全球家禽业造成了巨大的经济损失，并威胁到人类健康。可变剪接（AS）是一种普遍的转录后调控机制，它可以从单个基因同时产生许多蛋白质，参与各种疾病和个体发育。在此，我们通过高通量 RNA 测序技术对野生型巨噬细胞（WT）和 APEC 感染巨噬细胞（APEC）中的全基因组 AS 事件进行了表征。在 APEC 与 WT 的比较中，共鉴定出 751 个差异表达（DE）AS 基因，包括 587 个 SE、114 个 MXE、25 个 RI、17 个 A3 和 8 个 A5 事件。功能分析表明，这些鉴定出的 DE AS 基因参与了“内吞作用”、“p53 信号通路”、“MAPK 信号通路”、“NOD 样受体信号通路”、“泛素介导的蛋白水解”和“黏附斑”等免疫相关途径。总之，我们全面研究了 APEC 感染过程中的 AS 事件。本研究扩展了我们对 APEC 感染过程的理解，为进一步治疗 APEC 感染提供了新的思路。
