College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
School of Biological and Chemical Engineering, Yangzhou Polytechnic College, Yangzhou, 225009, China.
BMC Vet Res. 2024 Feb 7;20(1):49. doi: 10.1186/s12917-024-03890-7.
Avian pathogenic E. coli (APEC) can cause localized or systemic infections, collectively known as avian colibacillosis, resulting in huge economic losses to poultry industry globally per year. In addition, increasing evidence indicates that long non-coding RNAs (lncRNAs) play a critical role in regulating host inflammation in response to bacterial infection. However, the role of lncRNAs in the host response to APEC infection remains unclear.
Here, we found 816 differentially expressed (DE) lncRNAs and 1,798 DE mRNAs in APEC infected chicken macrophages by RNAseq. The identified DE lncRNA-mRNAs were involved in Toll like receptor signaling pathway, VEGF signaling pathway, fatty acid metabolism, phosphatidylinositol signaling system, and other types of O-glycan biosynthesis. Furthermore, we found the novel lncRNA TCONS_00007391 as an important immune regulator in APEC infection was able to regulate the inflammatory response by directly targeting CD86.
These findings provided a better understanding of host response to APEC infection and also offered the potential drug targets for therapy development against APEC infection.
禽致病性大肠杆菌（APEC）可引起局部或全身感染，统称为禽大肠杆菌病，每年给全球家禽业造成巨大的经济损失。此外，越来越多的证据表明，长非编码 RNA（lncRNA）在调节宿主对细菌感染的炎症反应中发挥着关键作用。然而，lncRNA 在宿主对 APEC 感染的反应中的作用尚不清楚。
通过 RNAseq，我们在 APEC 感染的鸡巨噬细胞中发现了 816 个差异表达的 lncRNA 和 1798 个差异表达的 mRNA。鉴定出的差异表达的 lncRNA-mRNA 参与 Toll 样受体信号通路、VEGF 信号通路、脂肪酸代谢、磷酸肌醇信号系统和其他类型的 O-聚糖生物合成。此外，我们发现新型 lncRNA TCONS_00007391 作为 APEC 感染中的一个重要免疫调节剂，能够通过直接靶向 CD86 来调节炎症反应。
这些发现加深了我们对宿主对 APEC 感染的反应的理解，并为开发针对 APEC 感染的治疗药物提供了潜在的靶点。
