小鼠结直肠癌类器官及成纤维细胞 - 类器官共培养物的整体染色
Whole-mount staining of mouse colorectal cancer organoids and fibroblast-organoid co-cultures.
作者信息
Martinez-Ordoñez Anxo, Cid-Diaz Tania, Duran Angeles, Han Qixiu, Moscat Jorge, Diaz-Meco Maria T
机构信息
Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA.
Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA.
出版信息
STAR Protoc. 2023 Apr 20;4(2):102243. doi: 10.1016/j.xpro.2023.102243.
Imaging organoid culture provides an excellent tool for studying complex diseases such as cancer. However, retaining the morphology of intact organoids for immunolabeling has been challenging. Here, we describe a protocol for immunofluorescence staining in intact colorectal cancer organoids derived from mice. We also describe additional steps for co-culture with mouse fibroblasts to enable the study of interactions with other cellular components of the tissue microenvironment. For complete details on the use and execution of this protocol, please refer to Martinez-Ordoñez et al. (2023)..
成像类器官培养为研究诸如癌症等复杂疾病提供了一个出色的工具。然而，保留完整类器官的形态用于免疫标记一直具有挑战性。在此，我们描述了一种对源自小鼠的完整结直肠癌类器官进行免疫荧光染色的方案。我们还描述了与小鼠成纤维细胞共培养的额外步骤，以能够研究与组织微环境中其他细胞成分的相互作用。有关此方案的使用和执行的完整详细信息，请参考马丁内斯 - 奥尔东涅斯等人（2023年）的研究。
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