Zhang Shaosen, Cheng Guoyu, Zhu Shihao, Lin Dongxin, Wu Chen
Department of Etiology and Carcinogenesis, National Cancer Center/National Clinical Research Center/Cancer Hospital, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing 100021, China.
Department of Etiology and Carcinogenesis, National Cancer Center/National Clinical Research Center/Cancer Hospital, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing 100021, China.
STAR Protoc. 2024 Dec 20;5(4):103316. doi: 10.1016/j.xpro.2024.103316. Epub 2024 Sep 14.
Multiple sampling strategies to cover different or dynamic stages of malignant continuum with organoid cultures provide a valuable platform for epithelium homeostasis, transformation, and cancer progression. Here, we present a protocol to initiate, culture, passage, and characterize organoids from normal and cancer-prone human esophageal tissues. We describe steps for multiple sampling of malignant continuum and the initiation and maintenance of multi-stage organoids. We then detail procedures for the histological characterization of organoids and co-culture systems based on organoids and stromal cells. For complete details on the use and execution of this protocol, please refer to Chen et al..
利用类器官培养覆盖恶性连续体不同或动态阶段的多种采样策略，为上皮内稳态、转化和癌症进展提供了一个有价值的平台。在这里，我们展示了一种从正常和易患癌的人类食管组织中起始、培养、传代和表征类器官的方案。我们描述了对恶性连续体进行多次采样以及起始和维持多阶段类器官的步骤。然后，我们详细介绍了基于类器官和基质细胞的类器官组织学表征及共培养系统的程序。有关本方案使用和执行的完整详细信息，请参阅Chen等人的研究。
