用于病毒转导和整体 3D 成像的小鼠输卵管上皮类器官生成方案。

Rosalind and Morris Goodman Cancer Institute, McGill University, Department of Human Genetics, 1160 Pine Avenue West, Montreal, QC H3A 1A3, Canada.

STAR Protoc. 2022 Feb 9;3(1):101164. doi: 10.1016/j.xpro.2022.101164. eCollection 2022 Mar 18.

Epithelial cells lining the oviduct/fallopian tube are essential in reproduction and have been identified as the cell-of-origin in high-grade serous ovarian carcinoma (HGSOC). This protocol describes the generation of organoids from mouse oviduct epithelial cells, providing a powerful tool to study epithelial homeostasis and malignant transformation. We also outline a protocol for whole-mount immunofluorescence and 3D confocal imaging. In addition, we describe approaches of viral transduction to investigate gene function in organoid development and epithelial cell behavior. For complete details on the use and execution of this profile, please refer to Ford et al. (2021).

输卵管/卵丘细胞是生殖过程中的重要细胞，已被鉴定为高级别浆液性卵巢癌（HGSOC）的起源细胞。本方案描述了从小鼠输卵管上皮细胞生成类器官的方法，为研究上皮细胞稳态和恶性转化提供了有力工具。我们还概述了用于全组织免疫荧光和 3D 共聚焦成像的方案。此外，我们还描述了病毒转导方法，用于研究基因功能在类器官发育和上皮细胞行为中的作用。有关该方案使用和实施的完整详细信息，请参考 Ford 等人（2021 年）。