PPP1R81 与低级别胶质瘤的生存和细胞增殖相关。

PPP1R81 correlates with the survival and cell proliferation in lower-grade glioma.

机构信息

Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang, China.

Jiangxi Key Laboratory of Neurological Tumors and Cerebrovascular Diseases, Nanchang, China.

出版信息

Biosci Rep. 2023 May 5;43(5). doi: 10.1042/BSR20230028.

DOI:10.1042/BSR20230028

PMID:37083601

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10170297/

Abstract

BACKGROUND

The specific functions of PPP1R81 has been elucidated in multiple cancers; however, its role in lower-grade glioma (LGG) remains unknown. In this research, we inspected the specific role of PPP1R81 in LGG.

METHODS

We totally evaluated the expression pattern and prognostic role of PPP1R81 in multitudinous tumors. Subsequently, we systematically examined the connection between PPP1R81 expression and prognosis, clinical characteristics, biological functions, genetic variations, and immunological characteristics in LGG according to the Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Altas (CGGA) databases. In vitro experiments were executed to inspect the expression level and specific roles of PPP1R81 in LGG.

RESULTS

PPP1R81 was elevated in multiple tumors and was tightly linked to a poor prognosis. LGG with higher expression of PPP1R81 showed poorer prognosis compared with lower expression of PPP1R81. The results of univariate and multivariate Cox regression analyses confirmed that the expression of PPP1R81 was an independent prognostic biomarker of LGG. Immune cell infiltration, immune checkpoint genes (ICPGs), copy number alterations (CNA), and tumor mutation burden (TMB) were also closely associated with PPP1R81 expression in LGG. In vitro experiments demonstrated that PPP1R81 was up-regulated and closely interrelated with cell proliferation and cell cycle in LGG.

CONCLUSION

PPP1R81 was an independent prognostic signature and underlying therapeutic target for patients with LGG.

摘要

背景

PPP1R81 的具体功能已在多种癌症中阐明；然而，其在低级别胶质瘤（LGG）中的作用尚不清楚。在这项研究中，我们检查了 PPP1R81 在 LGG 中的特定作用。

方法

我们全面评估了 PPP1R81 在多种肿瘤中的表达模式和预后作用。随后，我们根据癌症基因组图谱（TCGA）和中国胶质瘤基因组图谱（CGGA）数据库，系统地检查了 PPP1R81 表达与 LGG 预后、临床特征、生物学功能、遗传变异和免疫特征之间的关系。进行体外实验以检查 PPP1R81 在 LGG 中的表达水平和特定作用。

结果

PPP1R81 在多种肿瘤中上调，与预后不良密切相关。PPP1R81 表达较高的 LGG 与 PPP1R81 表达较低的 LGG 相比，预后较差。单因素和多因素 Cox 回归分析的结果证实，PPP1R81 的表达是 LGG 的独立预后生物标志物。免疫细胞浸润、免疫检查点基因（ICPGs）、拷贝数改变（CNA）和肿瘤突变负担（TMB）也与 LGG 中 PPP1R81 的表达密切相关。体外实验表明，PPP1R81 在 LGG 中上调，并与细胞增殖和细胞周期密切相关。