Hosseini Fatemeh Sadat, Kharazmi Farbod, Davarani Saied Saeed Hosseiny, Ebrahimzadeh Homeira
Department of Analytical Chemistry and Pollutants, Faculty of Chemistry and Petroleum Sciences, Shahid Beheshti University, Tehran, Iran.
Department of Analytical Chemistry and Pollutants, Faculty of Chemistry and Petroleum Sciences, Shahid Beheshti University, Tehran, Iran.
J Chromatogr A. 2023 May 24;1697:463984. doi: 10.1016/j.chroma.2023.463984. Epub 2023 Apr 12.
Today, antidepressants are widely used and it is important to determine their trace amounts due to harmful consequences. Here, a new nano sorbent was reported for the simultaneous extraction and determination of three types of antidepressant drugs (Clomipramine (CLO), Clozapine (CLZ), and Trimipramine (TRP) by the thin-film solid-phase micro-extraction (TFME-μSPE) method followed by the Gas Chromatography-flame ionization detector (GC-FID) analysis. So, the compound poly (vinyl alcohol) (PVA)/citric acid(CA)/β-cyclodextrin/BiS@g-CN nano sorbent was constructed by electrospinning technique. Then, nano sorbent was studied to optimize the many parameters impacting the extraction performance. Electrospun nanofiber has a large surface area, high porosity, and homogeneous morphology with a uniform bead-free structure. In optimal conditions, the limits of detection and quantification were calculated to be 0.15-0.03 ng mL and 0.5-0.1 ng mL, respectively. The dynamic linear range (DLR) was in the range of 0.1 to 1000 ng mL for CLO and CLZ, and 0.5 to 1000 ng mL for TRP with correlation coefficients (R) of 0.999. The relative standard deviations (RSDs) were achieved in the range of 4.9-6.8% (intra-day, n = 4) and 5.4-7.9% (inter-day, n = 3) in the period of 3 days. Finally, the capability of the method was evaluated to simultaneously measure trace amounts of antidepressants aqueous sample with desirable extraction efficiency (78 to 95%).
如今，抗抑郁药被广泛使用，由于其有害后果，测定其痕量至关重要。在此，报道了一种新型纳米吸附剂，用于通过薄膜固相微萃取（TFME-μSPE）方法同时萃取和测定三种抗抑郁药物（氯米帕明（CLO）、氯氮平（CLZ）和曲米帕明（TRP）），随后进行气相色谱-火焰离子化检测器（GC-FID）分析。因此，通过静电纺丝技术构建了复合聚乙烯醇（PVA）/柠檬酸（CA）/β-环糊精/BiS@g-CN纳米吸附剂。然后，对纳米吸附剂进行了研究，以优化影响萃取性能的诸多参数。静电纺纳米纤维具有大表面积、高孔隙率和均匀的形态以及无珠均匀结构。在最佳条件下，计算出的检测限和定量限分别为0.15 - 0.03 ng/mL和0.5 - 0.1 ng/mL。CLO和CLZ的动态线性范围（DLR）为0.1至1000 ng/mL，TRP为0.5至1000 ng/mL，相关系数（R）为0.999。在3天的时间内，相对标准偏差（RSD）在4.9 - 6.8%（日内，n = 4）和5.4 - 7.9%（日间，n = 3）范围内。最后，评估了该方法同时测量痕量抗抑郁药水样的能力，萃取效率良好（78%至95%）。
