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神经中胚层聚集体在体外再现了周围神经系统发育的某些方面。

Neuro-mesodermal assembloids recapitulate aspects of peripheral nervous system development in vitro.

机构信息

Institute of Anatomy and Cell Biology, University of Würzburg, Koellikerstraße 6, 97070 Würzburg, Germany.

Institute of Anatomy and Cell Biology, University of Würzburg, Koellikerstraße 6, 97070 Würzburg, Germany.

出版信息

Stem Cell Reports. 2023 May 9;18(5):1155-1165. doi: 10.1016/j.stemcr.2023.03.012. Epub 2023 Apr 20.

DOI:10.1016/j.stemcr.2023.03.012
PMID:37084722
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10202657/
Abstract

Here we describe a novel neuro-mesodermal assembloid model that recapitulates aspects of peripheral nervous system (PNS) development such as neural crest cell (NCC) induction, migration, and sensory as well as sympathetic ganglion formation. The ganglia send projections to the mesodermal as well as neural compartment. Axons in the mesodermal part are associated with Schwann cells. In addition, peripheral ganglia and nerve fibers interact with a co-developing vascular plexus, forming a neurovascular niche. Finally, developing sensory ganglia show response to capsaicin indicating their functionality. The presented assembloid model could help to uncover mechanisms of human NCC induction, delamination, migration, and PNS development. Moreover, the model could be used for toxicity screenings or drug testing. The co-development of mesodermal and neuroectodermal tissues and a vascular plexus along with a PNS allows us to investigate the crosstalk between neuroectoderm and mesoderm and between peripheral neurons/neuroblasts and endothelial cells.

摘要

在这里，我们描述了一种新型的神经中胚层集合模型，该模型再现了周围神经系统 (PNS) 发育的某些方面，如神经嵴细胞 (NCC) 的诱导、迁移以及感觉和交感神经节的形成。神经节向中胚层和神经区发送投射。中胚层部分的轴突与施万细胞相关。此外，周围神经节和神经纤维与共同发育的血管丛相互作用，形成神经血管龛。最后，发育中的感觉神经节对辣椒素表现出反应，表明其具有功能性。所提出的集合模型可以帮助揭示人类 NCC 诱导、分层、迁移和 PNS 发育的机制。此外，该模型可用于毒性筛选或药物测试。中胚层和神经外胚层组织以及血管丛的共同发育以及 PNS 的存在，使我们能够研究神经外胚层和中胚层之间以及周围神经元/神经母细胞和内皮细胞之间的串扰。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/7e719625c1e4/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/6a089823b58f/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/b138542a2060/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/0caee15d8670/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/bbd0e87edeac/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/b0b716b78662/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/782233c9cbf5/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/7e719625c1e4/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/6a089823b58f/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/b138542a2060/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/0caee15d8670/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/bbd0e87edeac/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/b0b716b78662/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/782233c9cbf5/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c0a/10202657/7e719625c1e4/gr6.jpg

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