膳食 neoagarotetraose 通过调节微生物群-肠道-大脑轴延长小鼠寿命并阻碍大脑衰老。
Dietary neoagarotetraose extends lifespan and impedes brain aging in mice via regulation of microbiota-gut-brain axis.
机构信息
Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
Department of Nutrition and Health, College of Engineering, China Agricultural University, Beijing, China.
出版信息
J Adv Res. 2023 Oct;52:119-134. doi: 10.1016/j.jare.2023.04.014. Epub 2023 Apr 20.
INTRODUCTION
Dietary oligosaccharides can impact the gut microbiota and confer tremendous health benefits.
OBJECTIVES
The aim of this study was to determine the impact of a novel functional oligosaccharide, neoagarotetraose (NAT), on aging in mice.
METHODS
8-month-old C57BL/6J mice as the natural aging mice model were orally administered with NAT for 12 months. The preventive effect of NAT in Alzheimer's disease (AD) mice was further evaluated. Aging related indicators, neuropathology, gut microbiota and short-chain fatty acids (SCFAs) in cecal contents were analyzed.
RESULTS
NAT treatment extended the lifespan of these mice by up to 33.3 %. Furthermore, these mice showed the improved aging characteristics and decreased injuries in cerebral neurons. Dietary NAT significantly delayed DNA damage in the brain, and inhibited reduction of tight junction protein in the colon. A significant increase at gut bacterial genus level (such as Lactobacillus, Butyricimonas, and Akkermansia) accompanied by increasing concentrations of SCFAs in cecal contents was observed after NAT treatment. Functional profiling of gut microbiota composition indicated that NAT treatment regulated the glucolipid and bile acid-related metabolic pathways. Interestingly, NAT treatment ameliorated cognitive impairment, attenuated amyloid-β (Aβ) and Tau pathology, and regulated the gut microbiota composition and SCFAs receptor-related pathway of Alzheimer's disease (AD) mice.
CONCLUSION
NAT mitigated age-associated cerebral injury in mice through gut-brain axis. The findings provide novel evidence for the effect of NAT on anti-aging, and highlight the potential application of NAT as an effective intervention against age-related diseases.
简介
膳食低聚糖可以影响肠道微生物群，并带来巨大的健康益处。
目的
本研究旨在确定一种新型功能性低聚糖 neoagarotetraose（NAT）对小鼠衰老的影响。
方法
以 8 月龄 C57BL/6J 小鼠为自然衰老小鼠模型，口服给予 NAT 12 个月。进一步评估 NAT 在阿尔茨海默病（AD）小鼠中的预防作用。分析衰老相关指标、神经病理学、肠道微生物群和盲肠内容物中的短链脂肪酸（SCFAs）。
结果
NAT 治疗使这些小鼠的寿命延长了 33.3%。此外，这些小鼠表现出改善的衰老特征和减少大脑神经元损伤。饮食 NAT 显著延缓了大脑中的 DNA 损伤，并抑制了结肠中紧密连接蛋白的减少。NAT 治疗后，肠道细菌属水平显著增加（如乳杆菌、丁酸单胞菌和阿克曼氏菌），盲肠内容物中的 SCFAs 浓度也显著增加。肠道微生物群组成的功能分析表明，NAT 治疗调节了糖脂和胆汁酸相关的代谢途径。有趣的是，NAT 治疗改善了认知障碍，减轻了淀粉样β（Aβ）和 Tau 病理学，并调节了 AD 小鼠的肠道微生物群组成和 SCFAs 受体相关途径。
结论
NAT 通过肠脑轴减轻了小鼠与年龄相关的大脑损伤。这些发现为 NAT 对抗衰老的影响提供了新的证据，并强调了 NAT 作为有效干预与年龄相关疾病的潜在应用。
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