Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
J Ethnopharmacol. 2023 Oct 5;314:116397. doi: 10.1016/j.jep.2023.116397. Epub 2023 Apr 20.
Qilong capsule (QLC) is a compound traditional Chinese medicine commonly used to treat ischemic stroke (IS). QLC is made of eight kinds of medicinal materials. It has two kinds of monarch medicine and six kinds of minister medicine. However, the pharmacodynamic mechanism of QLC is still unknown.
The aim of this paper was to evaluate the pharmacology mechanism of QLC against ischemic stroke through coagulation, inflammation and apoptosis.
We used an existing zebrafish model to explore the protective mechanism of QLC on IS. We treated normally-developing zebrafish larvae with QLC and ponatinib 2 days post fertilization (dpf), and used the area of cerebral vascular thrombosis, red blood cell staining intensity, and brain cell apoptosis to quantitate QLC efficacy against IS. Evaluation of brain inflammation in zebrafish by observing macrophage aggregation and migration. In addition, we also explored the effect of QLC on zebrafish angiogenesis. Quantitative polymerase chain reaction (qPCR) was used to detect changes in the expression of genes involved in coagulation, inflammation, vascular endothelium, and apoptosis.
We found that QLC reduced the area affected by ponatinib-induced cerebral vascular embolism, erythrocyte staining intensity, and the number of apoptotic brain cells. For inflammation, QLC can improve the aggregation and migration of macrophages. QLC can significantly promote the formation of blood vessels in zebrafish. qPCR showed that QLC inhibited the expression of genes related to coagulation, inflammation, and apoptosis.
Qilong capsule had a significant protective efficacy in ponatinib-induced IS.
芪龙胶囊（QLC）是一种常用于治疗缺血性中风（IS）的复方中药。QLC 由八种药材组成，有两种君药和六种臣药。然而，QLC 的药效学机制尚不清楚。
本文旨在通过凝血、炎症和细胞凋亡来评估 QLC 治疗缺血性中风的药理学机制。
我们使用现有的斑马鱼模型来探索 QLC 对 IS 的保护机制。我们在受精后 2 天（dpf）用 QLC 和 ponatinib 处理正常发育的斑马鱼幼虫，并通过测量脑血管血栓形成面积、红细胞染色强度和脑细胞凋亡来定量评估 QLC 对 IS 的疗效。通过观察巨噬细胞聚集和迁移来评估斑马鱼脑内炎症。此外，我们还探索了 QLC 对斑马鱼血管生成的影响。采用定量聚合酶链反应（qPCR）检测与凝血、炎症、血管内皮和细胞凋亡相关的基因表达变化。
我们发现 QLC 减少了 ponatinib 诱导的脑血管栓塞、红细胞染色强度和脑细胞凋亡的面积。对于炎症，QLC 可以改善巨噬细胞的聚集和迁移。QLC 能显著促进斑马鱼血管的形成。qPCR 显示 QLC 抑制了与凝血、炎症和细胞凋亡相关的基因表达。
芪龙胶囊对 ponatinib 诱导的 IS 具有显著的保护作用。
