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来自[具体来源未提及]的丹皮酚、香叶木素-7-O-葡萄糖苷和5-羟甲基糠醛的组合可减轻斑马鱼模型中花生四烯酸诱导的血栓形成。

The combination of paeonol, diosmetin-7----glucopyranoside, and 5-hydroxymethylfurfural from alleviates arachidonic acid-induced thrombosis in a zebrafish model.

作者信息

Lin Shenghua, Ma Honglin, Zhang Shanshan, Fan Wei, Shen Chuanlin, Chen Jiayu, Jin Meng, Li Kun, He Qiuxia

机构信息

Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.

Department of Gastroenterology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan, China.

出版信息

Front Pharmacol. 2024 Feb 29;15:1332468. doi: 10.3389/fphar.2024.1332468. eCollection 2024.

DOI:10.3389/fphar.2024.1332468
PMID:38487165
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10937350/
Abstract

(TF) is a classic medicinal material obtained from Shandong, China. The peel of this fruit (, TP) is known to exert anti-thrombotic effects. However, the anti-thrombotic active components and mechanisms of TP have yet to be fully elucidated. Combined with zebrafish models and high-performance liquid chromatography (HPLC), this study evaluated the endogenous anti-thrombotic effects with the combination of three compounds from TP. First, we used HPLC to investigate the components in the water extract of TP. Next, we used the zebrafish model to investigate the anti-thrombotic activity of the three compound combinations by evaluating a range of indicators. Finally, the expression of related genes was detected by real-time quantitative polymerase chain reaction (qPCR). HPLC detected a total of eight components in TP water extract, with high levels of paeonol (Pae), diosmetin-7----glucopyranoside (diosmetin-7--glucoside), and 5-hydroxymethylfurfural (5-HMF). The most significant anti-thrombotic activity was detected when the Pae: diosmetin-7--glucoside:5-HMF ratio was 4:3:3. qPCR analysis revealed that the abnormal expression levels of , , , , , and induced by arachidonic acid (AA) were improved. The combination of Pae, diosmetin-7--glucoside, and 5-HMF may alleviate AA-induced thrombosis by inhibiting the inflammatory reaction, coagulation cascade reaction, and arachidonic acid metabolism pathways.

摘要

（TF）是一种源自中国山东的经典药材。已知这种水果的果皮（TP）具有抗血栓形成作用。然而，TP的抗血栓形成活性成分和机制尚未完全阐明。本研究结合斑马鱼模型和高效液相色谱法（HPLC），评估了TP中三种化合物组合的内源性抗血栓形成作用。首先，我们使用HPLC研究TP水提取物中的成分。接下来，我们使用斑马鱼模型，通过评估一系列指标来研究三种化合物组合的抗血栓形成活性。最后，通过实时定量聚合酶链反应（qPCR）检测相关基因的表达。HPLC在TP水提取物中共检测到8种成分，其中丹皮酚（Pae）、香叶木素-7-O-葡萄糖苷（diosmetin-7-O-glucoside）和5-羟甲基糠醛（5-HMF）含量较高。当Pae：香叶木素-7-O-葡萄糖苷：5-HMF的比例为4：3：3时，检测到最显著的抗血栓形成活性。qPCR分析显示，花生四烯酸（AA）诱导的、、、、、和的异常表达水平得到改善。Pae、香叶木素-7-O-葡萄糖苷和5-HMF的组合可能通过抑制炎症反应、凝血级联反应和花生四烯酸代谢途径来减轻AA诱导的血栓形成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/fe8ff5e3f9d4/fphar-15-1332468-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/9e0825e3721d/fphar-15-1332468-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/0aa9be005418/fphar-15-1332468-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/791a993e277f/fphar-15-1332468-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/d5412a3e0205/fphar-15-1332468-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/530d8f95790b/fphar-15-1332468-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/5ee05146efca/fphar-15-1332468-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/c3e0118ba6f8/fphar-15-1332468-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/fe8ff5e3f9d4/fphar-15-1332468-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/9e0825e3721d/fphar-15-1332468-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/0aa9be005418/fphar-15-1332468-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/791a993e277f/fphar-15-1332468-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/d5412a3e0205/fphar-15-1332468-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/530d8f95790b/fphar-15-1332468-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/5ee05146efca/fphar-15-1332468-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/c3e0118ba6f8/fphar-15-1332468-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd40/10937350/fe8ff5e3f9d4/fphar-15-1332468-g008.jpg

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