National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
Hanoi Medical University, Hanoi, Vietnam.
Am J Trop Med Hyg. 2022 Jun 13;107(1):65-71. doi: 10.4269/ajtmh.21-1203. Print 2022 Jul 13.
Plasmid-Mediated Colistin Resistance 1 (mcr-1) was first reported in 2015 and is a great concern to human health. In this study, we investigated the prevalence of mcr-1 and mcr-1-positive Escherichia coli (MCRPEC) and the association in infection status among various reservoirs connected to livestock. The study was conducted in 70 poultry and swine farms in a commune in Ha Nam province, northern Vietnam. Samples were collected from farmers, food animals, domestic animals, and farm environments (flies and wastewater) for polymerase chain reaction (PCR) screening for mcr-1 gene and species identification of PCR positive isolates. Among 379 obtained mcr-1 positives isolates, Escherichia coli was the major identified, varying from 50% (2/4) in dog feces to 100% (31/31) in humans feces isolates. The prevalence of MCRPEC was 14.4% (20/139), 49.7% (96/193), 31.3% (25/80), 36.7% (40/109), 26.9% (18/67), and 3.9% (2/51) in humans, chickens, pigs, flies, wastewater, and dogs, respectively. The study identified association between MCRPEC infection status in humans and flies (OR = 3.4), between flies and chickens (OR = 5.3), and between flies and pigs (OR = 9.0). Farmers' age and farm livestock unit were also associated factors of MCRPEC infection status in humans (OR = 5.1 and 1.05, respectively). These findings bring new knowledge on antibiotic resistance in livestock setting and important suggestions on potential role of flies in the transmission of mcr-1 resistance gene.
质粒介导的黏菌素耐药 1 型（mcr-1）于 2015 年首次报道，对人类健康构成巨大威胁。本研究旨在调查与家畜相关的各种宿主中 mcr-1 和 mcr-1 阳性大肠杆菌（MCRPEC）的流行情况及其感染状况的关联性。该研究在越南北部河静省一个公社的 70 个家禽和养猪场进行。从农民、食用动物、家畜和农场环境（苍蝇和废水）中采集样本，进行聚合酶链反应（PCR）筛选 mcr-1 基因，并对 PCR 阳性分离株进行种属鉴定。在 379 株获得的 mcr-1 阳性分离株中，大肠埃希菌是主要鉴定的菌种，从狗粪便中的 50%（2/4）到人类粪便中的 100%（31/31）不等。MCRPEC 的流行率在人类、鸡、猪、苍蝇、废水和狗中分别为 14.4%（20/139）、49.7%（96/193）、31.3%（25/80）、36.7%（40/109）、26.9%（18/67）和 3.9%（2/51）。研究发现，人类 MCRPEC 感染状况与苍蝇之间存在关联（OR=3.4），苍蝇与鸡之间存在关联（OR=5.3），苍蝇与猪之间存在关联（OR=9.0）。农民年龄和农场牲畜单位也是人类 MCRPEC 感染状况的相关因素（OR=5.1 和 1.05）。这些发现为家畜环境中的抗生素耐药性提供了新的知识，并对苍蝇在 mcr-1 耐药基因传播中的潜在作用提出了重要建议。
