State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Marine Sciences, Ningbo University, Ningbo 315211, China; School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
Sci Total Environ. 2023 Jul 20;883:163633. doi: 10.1016/j.scitotenv.2023.163633. Epub 2023 Apr 21.
A sensitive and rugged analytical method was first established to simultaneously determine 60 herbicides in aquatic products with gas chromatography-tandem mass spectroscopy (GC-MS/MS). After extraction with acetonitrile (MeCN), NaCl and anhydrous NaSO were added, concentrated supernatants were directly passed through the Carb/NH solid phase extraction column. Then, the cartridge was rinsed with elution solution (MeCN/toluene, 3:1, v/v), followed by GC-MS/MS analysis with multiple reaction monitoring. An excellent linearity (1.0-100.0 μg/L) with R value of ≥0.9991 was obtained, and the limits of quantification were 0.018-3.852 μg/kg. Satisfactory recoveries (70.8 %-117.6 %) with RSDs below 11.0 % of herbicide residues were obtained at spiked levels of 0.010-0.050 mg/kg. Furthermore, herbicide residues in actual aquatic products were analyzed, and the acute/chronic risk assessment of dietary exposure was carried out. The wide use of herbicides for controlling weed and removing moss and harmful algae may obviously increase the risk of contamination of the aquaculture environment and fishery products. Therefore, considerable attention and more research are necessary to monitor residue levels for herbicides in aquatic products and ensure the quality of marine products and consumer safety.
建立了一种灵敏、耐用的分析方法，首次采用气相色谱-串联质谱法（GC-MS/MS）同时测定水产品中的 60 种除草剂。经乙腈（MeCN）提取后，加入 NaCl 和无水 NaSO4，浓缩上清液直接通过 Carb/NH 固相萃取柱。然后用洗脱液（MeCN/甲苯，3:1，v/v）冲洗小柱，再采用多反应监测进行 GC-MS/MS 分析。得到了极好的线性（1.0-100.0μg/L，R 值≥0.9991），定量限为 0.018-3.852μg/kg。在 0.010-0.050mg/kg 的添加水平下，除草剂残留的回收率为 70.8%-117.6%，RSD 低于 11.0%。此外，还分析了实际水产品中的除草剂残留，并进行了膳食暴露的急性/慢性风险评估。广泛使用除草剂来控制杂草、去除苔藓和有害藻类，可能会明显增加水产养殖环境和渔业产品污染的风险。因此，有必要对水产品中的除草剂残留进行监测，并确保海产品的质量和消费者的安全，这需要引起相当的关注和进行更多的研究。
