Chetsanga C J, Mavunga I
Chem Biol Interact. 1986 Apr;58(1):117-23. doi: 10.1016/s0009-2797(86)80091-6.
Imidazole ring opened adenine and guanine residues similar to those generated by gamma-irradiation of nucleosides of DNA, were chemically synthesised. Reaction conditions that promote the chemical reclosure of opened imidazole rings of guanine have been identified. The optimal conditions for the reclosure of such rings was found to be 0.2 M HCl at 37 degrees C. These conditions did not promote a reclosure of opened imidazole rings of adenine. The reclosure of opened imidazole rings of guanine was found to follow first order kinetics. The very low pH for this chemical ring reclosure precludes the likelihood that this reaction occurs intracellularly.
合成了与 DNA 核苷经γ射线辐照产生的类似的咪唑环打开的腺嘌呤和鸟嘌呤残基。已经确定了促进鸟嘌呤打开的咪唑环化学重新闭合的反应条件。发现此类环重新闭合的最佳条件是在 37℃下 0.2M 的盐酸。这些条件不会促进腺嘌呤打开的咪唑环的重新闭合。发现鸟嘌呤打开的咪唑环的重新闭合遵循一级动力学。这种化学环重新闭合所需的极低 pH 排除了该反应在细胞内发生的可能性。
