State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, China.
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, China.
Food Res Int. 2023 May;167:112668. doi: 10.1016/j.foodres.2023.112668. Epub 2023 Mar 21.
Aging of green tea leads to reductions in its flavor and health value, yet in situ testing methods for green tea freshness are lacking. A novel sensitive indicator displacement assay (IDA) sensor was constructed and applied for monitoring of green tea freshness during storage. Low-cost pH dyes and metal ions were used as indicators and receptors, respectively, for the targeted detection of catechins in tea samples. The feasibility of the IDA reaction was verified using images and UV-vis spectroscopy, respectively. IDA combined with supervised algorithms achieved accurate identification of green tea freshness with an accuracy of 86.67%, and acceptable accuracies in the prediction of catechin monomers and total catechins with ratio of prediction to deviation values over 1.5. Thus, the developed IDA sensor is capable of qualitative and quantitative monitoring of the green tea freshness during storage, providing a new option for quality evaluation and control of green teas.
绿茶的陈化会使其风味和健康价值降低，但缺乏原位测试绿茶新鲜度的方法。本研究构建了一种新颖的灵敏指示剂置换分析（IDA）传感器，并将其应用于储存过程中绿茶新鲜度的监测。低成本的 pH 染料和金属离子分别作为指示剂和受体，用于茶叶样品中儿茶素的靶向检测。IDA 反应的可行性分别通过图像和紫外可见光谱进行了验证。IDA 结合有监督算法实现了对绿茶新鲜度的准确识别，准确率为 86.67%，并且在预测儿茶素单体和总儿茶素方面具有可接受的准确性，预测值与偏差值之比超过 1.5。因此，所开发的 IDA 传感器能够定性和定量监测储存过程中绿茶的新鲜度，为绿茶的质量评价和控制提供了一种新的选择。
