转录因子定位动力学和 DNA 结合驱动不同的启动子解读。
Transcription factor localization dynamics and DNA binding drive distinct promoter interpretations.
机构信息
Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA; University of Wisconsin Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
出版信息
Cell Rep. 2023 May 30;42(5):112426. doi: 10.1016/j.celrep.2023.112426. Epub 2023 Apr 21.
Environmental information may be encoded in the temporal dynamics of transcription factor (TF) activation and subsequently decoded by gene promoters to enact stimulus-specific gene expression programs. Previous studies of this behavior focused on the encoding and decoding of information in TF nuclear localization dynamics, yet cells control the activity of TFs in myriad ways, including by regulating their ability to bind DNA. Here, we use light-controlled mutants of the yeast TF Msn2 as a model system to investigate how promoter decoding of TF localization dynamics is affected by changes in the ability of the TF to bind DNA. We find that yeast promoters directly decode the light-controlled localization dynamics of Msn2 and that the effects of changing Msn2 affinity on that decoding behavior are highly promoter dependent, illustrating how cells could regulate TF localization dynamics and DNA binding in concert for improved control of gene expression.
环境信息可能编码在转录因子 (TF) 激活的时间动态中，然后通过基因启动子进行解码，以实现刺激特异性基因表达程序。以前对这种行为的研究主要集中在 TF 核定位动力学中的信息编码和解码上，但细胞以多种方式控制 TF 的活性，包括调节它们与 DNA 结合的能力。在这里，我们使用酵母 TF Msn2 的光控突变体作为模型系统，研究启动子解码 TF 定位动力学如何受到 TF 结合 DNA 能力变化的影响。我们发现，酵母启动子直接解码 Msn2 的光控定位动力学，并且改变 Msn2 亲和力对该解码行为的影响高度依赖于启动子，这说明了细胞如何协调 TF 定位动力学和 DNA 结合，以实现对基因表达的更好控制。
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