School of Public Health, Nantong University, Nantong 226019, China.
School of Life Sciences, Nantong University, Nantong 226019, China.
J Pharm Biomed Anal. 2023 Jul 5;231:115389. doi: 10.1016/j.jpba.2023.115389. Epub 2023 Apr 12.
Among the analytical tools, paper-based analytical devices (PADs) have become a leading alternative for point-of care testing (POCT). In this study, PADs were fabricated using an office laser printer. Then, the paper zone was modified with graphene oxide (GO) and pyrene derivatives, which provide a sufficient amount of carboxylic groups for conjugating antibodies. At an optimal pH, antibodies were covalently bound onto carboxylated cellulose surface in an oriented manner through a two-step strategy: electrostatic adsorption was followed by EDC/NHS coupling. α-fetoprotein (AFP) as a detection model, we compared with cellulose powder modified and unmodified paper zone. The results showed the color intensity and color uniformity on GO modified paper was improved. The activity of immobilized antibodies on GO/1-pyrenebutyric acid (GO/PBA) modified was three times higher than that of GO modified and about 1.8-fold higher than that of GO/1-pyrenecarboxylic acid (GO/PCA) modified. The GO/PBA modified paper-based immunoassay has enhanced sensitivity and low detection limit. A linear correlation between color intensity and concentration of AFP in the range of 0.01～16.5 ng mL with a detection limit of 9.0 pg mL were achieved, respectively. The obtained results point towards rapid, sensitive, and specific early diagnosis of liver cancer at the point of care and other low-resource settings.
在分析工具中，纸基分析器件（PADs）已成为即时检测（POCT）的主要替代方法。在本研究中，使用办公激光打印机制造 PADs。然后，通过两步策略：静电吸附 followed by EDC/NHS coupling，使纸区用氧化石墨烯（GO）和芘衍生物修饰，这些衍生物提供了足够的羧基用于偶联抗体。在最佳 pH 值下，抗体通过静电吸附 followed by EDC/NHS coupling 以定向方式共价结合到羧基化纤维素表面上。以甲胎蛋白（AFP）作为检测模型，我们将其与纤维素粉末修饰和未修饰的纸区进行了比较。结果表明，GO 修饰的纸的颜色强度和颜色均匀性得到了改善。GO/1-芘丁酸（GO/PBA）修饰的固定化抗体的活性比 GO 修饰的高 3 倍，比 GO/1-芘羧酸（GO/PCA）修饰的高约 1.8 倍。GO/PBA 修饰的纸基免疫测定具有增强的灵敏度和低检测限。在 0.01～16.5ng/mL 的范围内，颜色强度与 AFP 浓度之间存在线性相关性，检测限为 9.0pg/mL。研究结果表明，在即时检测和其他资源匮乏的环境下，可以快速、灵敏、特异性地进行肝癌早期诊断。
