Chen Wenhui, Li Tingting, Chen Chengbo, Zhang Jinghui, Ma Ziyu, Hou Weilin, Yao Yao, Mao Wei, Liu Chang, Kong Dezhao, Tang Sheng, Shen Wei
School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212003, Jiangsu Province, PR China.
Department of Medicinal Chemistry, University of Washington, Seattle, WA, 98195, USA.
Biosens Bioelectron. 2023 Jul 15;232:115335. doi: 10.1016/j.bios.2023.115335. Epub 2023 Apr 17.
Precise and early screening of colorectal cancer (CRC) is one crucial yet challenging task for its treatment, and the analysis of multi-targets of CRC in a single assay with high accuracy is essential for pathological research and clinical diagnosis. Here, a CRC-related biomarker pair, microRNA-211 (miRNA-211) and HS, was detected by constructing a three-dimensional (3D) ordered DNA network. First, trace amount of miRNA-211 could initiate a hybridization chain reaction-based amplification process. A highly ordered 3D DNA network was formed based on the organized assembly of DNA-cube frameworks that were constructed by DNA origamis and Ag nanoparticles (NPs) encapsulated inside. In the presence of the HS, Ag NPs within the network can be etched to generate AgS quantum dots, which could be better visualized in fluorescence in situ cell imaging. Using the 3D DNA ordered network as the sensing platform, it can acquire dual analysis of biomolecule (miRNA-211) and inorganic gas (HS) in vitro, overcoming the limitations of single type of biomarker detection in a single assay. This assay achieved a wide linearity range of HS from 0.05 to 10 μM, and exhibited a low limit of detection of 4.78 nM. This strategy allows us to acquire the spatial distributions of HS and miRNA expression levels in living CRC cells simultaneously, providing a highly sensitive and selective tool for early screening and monitoring of CRC.
结直肠癌（CRC）的精准早期筛查是其治疗的一项关键但具有挑战性的任务，在单一检测中高精度分析CRC的多个靶点对于病理研究和临床诊断至关重要。在此，通过构建三维（3D）有序DNA网络检测了一对与CRC相关的生物标志物，即微小RNA-211（miRNA-211）和硫化氢（HS）。首先，痕量的miRNA-211可引发基于杂交链式反应的扩增过程。基于由DNA折纸和包裹在内部的银纳米颗粒（NPs）构建的DNA立方框架的有序组装，形成了高度有序的3D DNA网络。在HS存在的情况下，网络内的银纳米颗粒可被蚀刻生成硫化银量子点，其在荧光原位细胞成像中能得到更好的可视化。以3D DNA有序网络作为传感平台，可在体外实现对生物分子（miRNA-211）和无机气体（HS）的双重分析，克服了单一检测中单一类型生物标志物检测的局限性。该检测方法实现了HS从0.05到10 μM的宽线性范围，并呈现出4.78 nM的低检测限。此策略使我们能够同时获取HS的空间分布和活的CRC细胞中miRNA的表达水平，为CRC的早期筛查和监测提供了一种高灵敏度和选择性的工具。
