Fu Yongxin, Wang Jiawu, Hu Zhiya, Gou Yang, Li Yisen, Jiang Qing
Department of Urology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
J Oncol. 2023 Apr 8;2023:9645038. doi: 10.1155/2023/9645038. eCollection 2023.
Clear cell renal cell carcinoma (ccRCC) is the most common pathology type of renal cancer that has an abysmal prognosis. Although a crucial role for 7-methylguanosine modification in cancer cell development has been reported, its role in ccRCC remains uncertain. This study was conducted to determine the efficacy of predictive biomarkers based on m7G-related genes in ccRCC. Firstly, we extracted clinical data and gene expression profiles of ccRCC patients from publicly accessible databases. It identified that 22 of the m7G-related 34 genes were related to overall survival, and 5 of the 22 genes were significantly expressed differently in tumor tissues. Based on Lasso regression analysis, five optimal genes (CYFIP2, EIF4A1, NUDT1, NUDT10, and NUDT4) were chosen to build a new predictive risk model in the TCGA cohort. Validation was carried out with the E-MTAB-1980 cohort. Then, a prognostic nomogram was erected, including the m7G-related gene risk score, age, histological grade, and stage status. Further studies and analysis showed that immune cell infiltration might be associated with the m7G-related risk genes. In addition, the relationship between gene expression and drug response was evaluated by the Pearson correlation test. Therefore, the risk signature with five selected m7G-related genes may be a promising prognostic biomarker and contribute to standardized prognostic assessment for ccRCC.
透明细胞肾细胞癌（ccRCC）是肾癌最常见的病理类型，预后极差。尽管已有报道7-甲基鸟苷修饰在癌细胞发育中起关键作用，但其在ccRCC中的作用仍不确定。本研究旨在确定基于m7G相关基因的预测生物标志物在ccRCC中的疗效。首先，我们从公开可用的数据库中提取了ccRCC患者的临床数据和基因表达谱。研究发现，34个m7G相关基因中的22个与总生存期相关，其中5个基因在肿瘤组织中的表达存在显著差异。基于套索回归分析，选择了五个最佳基因（CYFIP2、EIF4A1、NUDT1、NUDT10和NUDT4）在TCGA队列中构建新的预测风险模型。并在E-MTAB-1980队列中进行了验证。然后，建立了一个预后列线图，包括m7G相关基因风险评分、年龄、组织学分级和分期状态。进一步的研究和分析表明，免疫细胞浸润可能与m7G相关风险基因有关。此外，通过Pearson相关性检验评估了基因表达与药物反应之间的关系。因此，由五个选定的m7G相关基因组成的风险特征可能是一种有前景的预后生物标志物，并有助于ccRCC的标准化预后评估。
