Abu-Sini Mohammad, Al-Kafaween Mohammad A, Al-Groom Rania M, Hilmi Abu Bakar Mohd
Department of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman, Jordan.
Department of Medical Laboratory Science, Faculty of Allied Medical Sciences, Zarqa University, Zarqa, Jordan.
AIMS Microbiol. 2023 Mar 30;9(2):313-331. doi: 10.3934/microbiol.2023017. eCollection 2023.
is an opportunistic pathogen that is commonly found in nosocomial infections. The purpose of this study was to investigate the effects of seven antibiotics on planktonic growth, biofilm formation, and the expression of virulence factors. These antibiotics included Ciprofloxacin (CP), Amikacin (AMK), Vancomycin (VAN), Tetracycline (TET), Gentamicin (GEN), Erythromycin (Ery), and Clindamycin (CLI). Antibiotic susceptibility testing, Minimum Bactericidal Concentration (MBC), Minimum Inhibitory Concentration (MIC), growth curve, time-kill curve, biofilm inhibition and reduction assay, and RT-qPCR were used to assess the effects of these antibiotics on planktonic and biofilm. The clear zones of inhibition against for the CP, AMK, VAN, TET, GEN, Ery, and CLI were 26 mm, 20 mm, 21 mm, 22 mm, 20 mm, 25 mm and 23 mm, respectively. The MIC values for CP, AMK, VAN, TET, GEN, Ery and CLI against ranged from 0.25 to 1 µg/mL while the MBC values ranged from 1 and 0.5 to 2 µg/mL respectively. The growth, total viable counts (TVCs), bacterial adhesion and biofilm formation of were reduced after exposure to all the tested antibiotics in a dose-dependent manner. The RT-qPCR analysis showed that all the tested antibiotics share a similar overall pattern of gene expression, with a trend toward reduced expression of the virulence genes of interest ( and ) in . The results indicate that all of the tested antibiotics possess antimicrobial and anti-biofilm activities, and that they may be multiple inhibitors and moderators of virulence via a variety of molecular targets. This deduction requires to be investigated .
是一种机会致病菌，常见于医院感染中。本研究的目的是调查七种抗生素对浮游生长、生物膜形成以及毒力因子表达的影响。这些抗生素包括环丙沙星（CP）、阿米卡星（AMK）、万古霉素（VAN）、四环素（TET）、庆大霉素（GEN）、红霉素（Ery）和克林霉素（CLI）。采用抗生素敏感性试验、最低杀菌浓度（MBC）、最低抑菌浓度（MIC）、生长曲线、时间杀菌曲线、生物膜抑制和减少试验以及逆转录定量聚合酶链反应（RT-qPCR）来评估这些抗生素对浮游菌和生物膜的影响。CP、AMK、VAN、TET、GEN、Ery和CLI对其的抑菌圈直径分别为26 mm、20 mm、21 mm、22 mm、20 mm、25 mm和23 mm。CP、AMK、VAN、TET、GEN、Ery和CLI对其的MIC值范围为0.25至1 μg/mL，而MBC值分别为1至0.5至2 μg/mL。暴露于所有测试抗生素后，其生长、总活菌数（TVC）、细菌黏附和生物膜形成均呈剂量依赖性降低。RT-qPCR分析表明，所有测试抗生素的基因表达总体模式相似，其感兴趣的毒力基因（和）在中的表达有降低趋势。结果表明，所有测试抗生素均具有抗菌和抗生物膜活性，并可能通过多种分子靶点成为其毒力的多种抑制剂和调节剂。这一推论有待进一步研究。
