National Centre for Nanoscience and Nanotechnology, University of Madras, Chennai 600025, India.
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai 600077, India.
Biomater Sci. 2023 May 30;11(11):3921-3937. doi: 10.1039/d3bm00169e.
The development of wound healing scaffolds with high porosity, rapid healing properties, and anti-inflammatory functionality is vital in the chronic wound healing stage for the production of extracellular matrices of injured tissues. The 45S5 bioactive glass (BG) possesses good biocompatibility and provides a potential bonding resource for fibroblast cell proliferation, growth factor synthesis, and granulated tissue formation. Chitosan, a natural polymer, promotes tissue regeneration and has anti-microbial properties. BG and chitosan scaffolds were prepared by the freeze-drying (lyophilization) method. The chitosan scaffold is a semi-crystalline polymer with a random crystal structure because it contains more hydroxyl groups. Chitosan alone shows a sheet-like morphology with a porous microstructure (1.7475 nm). BG particulates were well decorated over the surface of the chitosan scaffold with a homogeneous dispersion. Cell viability was observed for L929 cells on the chitosan-BG scaffolds. Confocal images vividly depict the interaction of the L929 cells with the scaffold without causing any damage to the cell membrane. scratch assay shows the best wound healing activity (complete wound closure) for the BG-chitosan nanocomposite scaffolds at 18 h. The chitosan-BG scaffolds were combined with anti-inflammatory drugs and induced inflammatory genes at an inhibition rate of COX of (36, 28, and 30%), LOX of (20, 13, and 14%), and NO of (48, 38, and 39%) for chitosan, chitosan-BG, and chitosan-BG (Na-free) at 100 μL addition. The bioactivities proved that the chitosan-BG scaffolds could enable better cell formation, and exhibited improved biocompatibility, and anti-inflammatory and wound healing properties.
具有高孔隙率、快速愈合特性和抗炎功能的伤口愈合支架的开发对于受伤组织的细胞外基质的产生至关重要。45S5 生物活性玻璃 (BG) 具有良好的生物相容性，并为成纤维细胞增殖、生长因子合成和肉芽组织形成提供了潜在的结合资源。壳聚糖是一种天然聚合物，可促进组织再生，具有抗菌性能。BG 和壳聚糖支架是通过冷冻干燥（冻干）法制备的。壳聚糖支架是一种半结晶聚合物，具有无定形晶体结构，因为它含有更多的羟基。壳聚糖单独显示出片状形态，具有多孔微结构（1.7475nm）。BG 颗粒很好地装饰在壳聚糖支架的表面上，具有均匀的分散性。在壳聚糖-BG 支架上观察到 L929 细胞的细胞活力。共聚焦图像生动地描述了 L929 细胞与支架的相互作用，而不会对细胞膜造成任何损伤。划痕试验表明，BG-壳聚糖纳米复合材料支架的愈合活性最佳（完全闭合伤口），在 18 小时内。壳聚糖-BG 支架与抗炎药物结合，以 COX（36、28 和 30%）、LOX（20、13 和 14%）和 NO（48、38 和 39%）的抑制率诱导炎症基因，对于壳聚糖、壳聚糖-BG 和壳聚糖-BG（无 Na）分别添加 100μL。生物活性证明，壳聚糖-BG 支架可以更好地促进细胞形成，并表现出更好的生物相容性、抗炎和伤口愈合性能。
