Division of Biosurface Technology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Poojappura, Thiruvananthapuram, Kerala, India.
Division of Biosurface Technology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Poojappura, Thiruvananthapuram, Kerala, India.
Int J Biol Macromol. 2023 Jul 1;242(Pt 1):124688. doi: 10.1016/j.ijbiomac.2023.124688. Epub 2023 May 1.
The present study reports the development of L-proline conjugated chitosan scaffold for wound healing application. Proline plays a beneficial role in collagen synthesis, and as a biochemical, it has the potential to modulate wound healing. In this regard, amino acid L-proline was conjugated onto chitosan, and the scaffolds were synthesised. FTIR and NMR analysis confirmed amino acid conjugation. The prepared scaffold was characterized by studies such as swelling, dissolution, tensile strength, porosity, water-vapor transmission rate and in-vitro healing properties. Cell viability assay showed that the scaffold has no cytotoxicity against the L929 and HaCaT cells. The in-vitro wound healing potential of the scaffold by scratch wound assay on the L929 cell line showed 53.35 ± 2.3 %, 72.96 ± 2.2 %, and 50.89 ± 0.3 % wound closure for CS-P 200, CS-P 400 and CS-P 600, respectively when compared to native CS scaffold (38.86 ± 1.6 %). A similar observation was found with HaCaT cells too. The studies showed that the modified scaffold promotes collagen deposition from fibroblast cells. These findings suggest that scaffold cues remodel the wound microenvironment for a better wound-healing state, and the L-proline conjugated scaffold may have considerable potential as a wound dressing to improve wound healing.
本研究报告了用于伤口愈合应用的 L-脯氨酸接枝壳聚糖支架的开发。脯氨酸在胶原蛋白合成中发挥有益作用，作为一种生物化学物质，它具有调节伤口愈合的潜力。在这方面，将氨基酸 L-脯氨酸接枝到壳聚糖上，并合成支架。FTIR 和 NMR 分析证实了氨基酸的接枝。通过溶胀、溶解、拉伸强度、孔隙率、水蒸气传输率和体外愈合性能等研究对制备的支架进行了表征。细胞活力测定表明，支架对 L929 和 HaCaT 细胞无细胞毒性。通过 L929 细胞系划痕实验评估支架的体外愈合潜力，与天然 CS 支架（38.86±1.6%）相比，CS-P200、CS-P400 和 CS-P600 支架的闭合率分别为 53.35±2.3%、72.96±2.2%和 50.89±0.3%。在 HaCaT 细胞中也观察到了类似的结果。这些研究表明，改性支架促进成纤维细胞的胶原蛋白沉积。这些发现表明支架提示重塑了伤口微环境，以达到更好的愈合状态，L-脯氨酸接枝支架作为一种改善伤口愈合的敷料具有相当大的潜力。
