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gen. nov., sp. nov., 一种新的家族成员,分离自西班牙西北部榆叶黑莓(Schott)的茎部。

gen. nov., sp. nov., a new member of family isolated from stems of elmleaf blackberry ( Schott) in Northwest Spain.

机构信息

Department of Microbiology and Genetics, University of Salamanca, Salamanca, Spain.

Institute of Investigation in Agrobiotechnology (CIALE), Salamanca, Spain.

出版信息

Int J Syst Evol Microbiol. 2023 Apr;73(4). doi: 10.1099/ijsem.0.005789.

Abstract

Strain CRRU44 was isolated from the stems of plants growing in Salamanca (Spain). The phylogenetic analysis of the 16S rRNA gene sequence places this strain within the family showing that it is equidistant to the type species of several genera from this family with similarity values ranging from 91.0 to 96.3 %. Strain CRRU44 formed a divergent lineage which clustered with RZME27, HAMBI540 and R1-200B4. The phylogenomic analysis showed that strain CRRU44 was equal to or more distant from the remaining genera of the family than other genera among them. The calculated average nucleotide identity based on blast and average amino acid identity values with respect to the type species of all genera from the family were lower than 78.5 and 76.5 %, respectively, which are the currently cut-off values proposed to differentiate genera within this family. All these results together with those from phenotypic and chemotaxonomic analyses support that strain CRRU44 represents a novel species of a novel genus within the family , for which the name gen. nov., sp. nov. is proposed (type strain CRRU44=CECT 30117=LMG 31822).

摘要

菌株 CRRU44 是从生长在西班牙萨拉曼卡的植物茎中分离出来的。16S rRNA 基因序列的系统发育分析将该菌株归入科,表明它与该科的几个属的模式种具有相同的距离,相似性值在 91.0%至 96.3%之间。菌株 CRRU44 形成了一个发散的谱系,与 RZME27、HAMBI540 和 R1-200B4 聚类。基因组系统发育分析表明,与该科的其他属相比,菌株 CRRU44 与科内其他属的距离相等或更远。基于 blast 的平均核苷酸同一性和相对于科内所有属的模式种的平均氨基酸同一性值计算,分别低于 78.5%和 76.5%,这是目前用于区分该科内属的截止值。所有这些结果与表型和化学分类学分析一起,支持菌株 CRRU44 代表科内一个新属的新种,建议使用新属名 ,新种名 (模式菌株 CRRU44=CECT 30117=LMG 31822)。

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