Microbiology Department, M. S. Swaminathan Research Foundation, 3rd Cross street, Taramani institutional area, Chennai 600113, India.
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central University, Gachibowli, Hyderabad 500046, India.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4484-4488. doi: 10.1099/ijs.0.049726-0. Epub 2013 Aug 1.
The taxonomic position of strain MSSRFBL1(T), isolated from chickpea rhizosphere soil from Kannivadi, India, was determined. Strain MSSRFBL1(T) formed bluish black colonies, stained Gram-negative and was motile, aerobic, capable of fixing dinitrogen, oxidase-negative and catalase-positive. Q-10 was the major respiratory quinone. Major fatty acids of strain MSSRFBL1(T) were C18 : 1ω7c and C19 : 0cycloω8c. Minor amounts of C18 : 0, C12 : 0, C14 : 0 3-OH, C18 : 0 3-OH, C16 : 0, C16 : 1ω6c/C16 : 1ω7c, C17 : 0 3-OH and C20 : 1ω7c were also present. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylcholine and two unidentified glycolipids. Bacteriohopane derivatives (BHD1 and 2), diplopterol, diploptene, bishomohopanediol, adenosylhopane and 2β-methyl bacteriohopanetetrol were the major hopanoids of strain MSSRFBL1(T). The genomic DNA G+C content was 71 mol%. EzTaxon-e-based blast analysis of the 16S rRNA gene indicated the highest similarity of strain MSSRFBL1(T) to Ensifer adhaerens LMG 20216(T) (97.3 %) and other members of the genus Ensifer (<96.9 %) in the family Rhizobiaceae of the class Alphaproteobacteria. However, phylogenetic analysis based on 16S rRNA, recA, thrC and dnaK gene sequences showed distinct out-grouping from the recognized genera of the family Rhizobiaceae. Based on phenotypic, genotypic and chemotaxonomic characters, strain MSSRFBL1(T) represents a novel species in a new genus in the family Rhizobiaceae for which the name Ciceribacter lividus gen. nov., sp. nov. is proposed. The type strain of Ciceribacter lividus is MSSRFBL1(T) ( = DSM 25528(T) = KCTC 32403(T)).
从印度 Kannivadi 的鹰嘴豆根际土壤中分离到 MSSRFBL1(T) 菌株，确定了其分类地位。 MSSRFBL1(T) 菌株形成蓝黑色菌落，革兰氏阴性，运动，需氧，能够固定二氮，氧化酶阴性，过氧化氢酶阳性。 Q-10 是主要的呼吸醌。 MSSRFBL1(T) 菌株的主要脂肪酸是 C18 : 1ω7c 和 C19 : 0cycloω8c。少量的 C18 : 0、C12 : 0、C14 : 0 3-OH、C18 : 0 3-OH、C16 : 0、C16 : 1ω6c/C16 : 1ω7c、C17 : 0 3-OH 和 C20 : 1ω7c 也存在。极性脂包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甲乙醇胺、磷脂酰胆碱和两种未鉴定的糖脂。细菌萜烷衍生物 (BHD1 和 2)、二孢醇、二孢烯、双霍烷二醇、腺苷霍烷和 2β-甲基细菌萜烷四醇是 MSSRFBL1(T) 菌株的主要霍烷类化合物。基因组 DNA G+C 含量为 71 mol%。基于 16S rRNA 基因的 EzTaxon-e blast 分析表明，MSSRFBL1(T) 菌株与根瘤菌属的 Ensifer adhaerens LMG 20216(T)（97.3%）和根瘤菌科的其他属成员（<96.9%）的相似度最高。然而，基于 16S rRNA、recA、thrC 和 dnaK 基因序列的系统发育分析表明，它与根瘤菌科公认的属明显不同。根据表型、基因型和化学分类特征，MSSRFBL1(T) 菌株代表了根瘤菌科一个新属的新种，建议将其命名为鹰嘴豆 Ciceribacter lividus 属。新种的模式菌株为 Ciceribacter lividus MSSRFBL1(T)（=DSM 25528(T)=KCTC 32403(T)）。
