Key Laboratory of Marine Genetic Resources, The Third Institute of Oceanography, Ministry of Natural Resources, State Key Laboratory Breeding Base of Marine Genetic Resources, Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China.
School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
Int J Syst Evol Microbiol. 2020 Oct;70(10):5488-5496. doi: 10.1099/ijsem.0.004439. Epub 2020 Sep 4.
A novel Gram-stain-negative, non-motile, strictly aerobic, rod-shaped bacterium was isolated from deep-sea hydrothermal sulfide in the northwest Indian Ocean Ridge and designated as strain IOP_32. Strain IOP_32 could grow at 4-40 °C (optimum, 37 °C), pH 5-9 (optimum, pH 7-8) and salinity of 0-12 % NaCl (w/v; optimum, 2-3 %). The 16S rRNA gene sequence of strain IOP_32 is most similar to EM7, RA3-3-1, KMM 6029 and S9_10 with 95.5-95.3 % similarity. The phylogenomic analysis indicated that strain IOP_32 forms a distinct lineage with Th78 within the family . The average nucleotide identity, average amino acid identity and percentage of conserved protein values between strain IOP_32 and the type strains of close genera were 72.3-78.5 %, 67.4-76.9 % and 56.3-61.6 %, respectively. The major cellular fatty acid was anteiso-C. The respiratory quinone was MK-6. The polar lipids were mainly composed of phosphatidylethanolamine, an unidentified aminophospholipid and five unidentified polar lipids. Strain IOP_32 is significantly different from related genera, which is reflected by the wide adaptability to temperature and salinity levels, the composition of phospholipids and fatty acids, and the results of phylogenetic analyses. The phenotypic properties and phylogenetic data suggest that the lineage represents a novel genus and species within the family , for which the name gen. sp. nov. is proposed, with the type strain IOP_32 (=MCCC 1A14017 =KCTC 62660 ). We also propose the reclassification of as comb. nov. (Th78=DSM 26285=JCM 18634=KCTC 32142).
一株新型革兰氏阴性、非运动、严格需氧、杆状细菌从西北印度洋脊的深海热液硫化物中分离得到，命名为 IOP_32 株。IOP_32 株可在 4-40°C（最适温度 37°C）、pH 5-9（最适 pH 7-8）和 0-12%NaCl（w/v；最适 2-3%）的盐度下生长。IOP_32 株的 16S rRNA 基因序列与 EM7、RA3-3-1、KMM 6029 和 S9_10 最为相似，相似度为 95.5-95.3%。系统发育基因组分析表明，IOP_32 株与. 科内的 Th78 形成一个独特的进化枝。与近缘属的模式菌株相比，IOP_32 株的平均核苷酸同一性、平均氨基酸同一性和保守蛋白百分比分别为 72.3-78.5%、67.4-76.9%和 56.3-61.6%。主要的细胞脂肪酸为 anteiso-C。呼吸醌为 MK-6。极性脂主要由磷脂酰乙醇胺、一种未鉴定的氨基磷脂和五种未鉴定的极性脂组成。IOP_32 株与相关属的菌株有明显的不同，这反映在其对温度和盐度水平的广泛适应性、磷脂和脂肪酸的组成以及系统发育分析的结果上。表型特征和系统发育数据表明，该谱系代表了. 科内的一个新属和新种，建议命名为 gen. sp. nov.，其模式菌株为 IOP_32 株（=MCCC 1A14017 =KCTC 62660）。我们还建议将. 重新分类为. comb. nov.（Th78=DSM 26285=JCM 18634=KCTC 32142）。
