Frazier J M
Environ Health Perspect. 1986 Mar;65:39-43. doi: 10.1289/ehp.866539.
Inducible cadmium-binding proteins (Cd-BP) in the mussel, Mytilus edulis, were resolved into two molecular weight components, designated Cd-BP10 and Cd-BP20, by gel-permeation chromatography on Sephadex G-75. Each of these two molecular weight components were further resolved into four subcomponents by DEAE-ion-exchange chromatography. All eight subcomponents bound cadmium and exhibited significant UV absorption at 254 nm and little absorption at 280 nm. Each subcomponent was purified and subjected to amino acid composition analysis. Two classes were identified, one having higher cysteine (23.9-26.6 mole-%) and lower glutamic acid contents compared to the other class (11.6-18.2 mole-% cysteine). All subcomponents have a relatively high glycine content (approximately 15 mole-%) relative to mammalian metallothioneins (approximately 8 mole-%). Although the Cd-BP20 have apparent molecular weights almost twice the Cd-BP10, the exact molecular relationship between these binding proteins is not known.
通过在葡聚糖凝胶G - 75上进行凝胶渗透色谱法，将贻贝（紫贻贝）中的可诱导镉结合蛋白（Cd - BP）分离为两个分子量组分，分别命名为Cd - BP10和Cd - BP20。通过DEAE - 离子交换色谱法，这两个分子量组分中的每一个又进一步分离为四个亚组分。所有八个亚组分都能结合镉，并在254 nm处表现出显著的紫外吸收，而在280 nm处吸收很少。对每个亚组分进行纯化并进行氨基酸组成分析。鉴定出两类，一类与另一类相比具有更高的半胱氨酸含量（23.9 - 26.6摩尔%）和更低的谷氨酸含量（11.6 - 18.2摩尔%半胱氨酸）。相对于哺乳动物金属硫蛋白（约8摩尔%），所有亚组分都具有相对较高的甘氨酸含量（约15摩尔%）。尽管Cd - BP20的表观分子量几乎是Cd - BP10的两倍，但这些结合蛋白之间的确切分子关系尚不清楚。
