紫贻贝（Mytilus edulis (L.)）中两种分子量镉结合蛋白的特性分析。

Characterization of two molecular weight classes of cadmium binding proteins from the mussel, Mytilus edulis (L.).

作者信息

Frazier J M, George S S, Overnell J, Coombs T L, Kagi J

出版信息

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;80(2):257-62. doi: 10.1016/0742-8413(85)90052-0.

Abstract

Inducible cadmium binding proteins (Cd-BP) in the mussel, Mytilus edulis, were resolved into two molecular weight components by gel permeation chromatography on Sephadex G-75. Each of these two molecular weight components was further resolved into four subcomponents by DEAE ion exchange chromatography. All eight subcomponents bound cadmium and exhibited significant u.v. absorption at 254 and little absorption at 280 nm. Based on amino acid composition analysis two classes of proteins were identified, one having higher cysteine (approximately 25 mole %) and lower serine and glutamic acid contents compared to the other class.

摘要

贻贝（紫贻贝）中的可诱导镉结合蛋白（Cd-BP）通过在葡聚糖凝胶G-75上进行凝胶渗透色谱法分离为两个分子量组分。这两个分子量组分中的每一个通过DEAE离子交换色谱法进一步分离为四个亚组分。所有八个亚组分都结合镉，并在254nm处表现出显著的紫外吸收，在280nm处吸收很少。基于氨基酸组成分析，鉴定出两类蛋白质，一类与另一类相比具有更高的半胱氨酸含量（约25摩尔%）以及更低的丝氨酸和谷氨酸含量。