School of Pharmaceutical and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan, 528400, China.
Guangzhou Vocational University of Science and Technology, Guangzhou, 510555, China.
Sci Rep. 2023 Apr 24;13(1):6640. doi: 10.1038/s41598-023-33661-7.
In this paper, a novel composite is constructed as a non-enzymatic hydrogen peroxide (HO) sensor by liquid-phase exfoliation method, which is composed of copper oxide, cuprous oxide and silver nanoparticles doped few-layer-graphene (CuO/Ag@FLG). Its surface morphology and composition were characterized by scanning electron microscopy (SEM) and X-ray photo spectroscopy (XPS), and its HO sensing performances include catalytic reduction and quantitative detection were studied with electrochemical methods. Our sensor had a high sensitivity of 174.5 μA mM cm (R = 0.9978) in an extremely wide range of concentrations from 10 μM to 100 mM, a fast response (about 5 s) and a low limit of detection (S/N = 3) of 2.13 μM. The sensor exhibits outstanding selectivity in the presence of various biological interference, such as dopamine, ascorbic acid, uric acid, citric acid, etc. In addition, the constructed sensor continued 95% current responsiveness after 1 month of storage further points to its long-term stability. Last but not least, it has a good recovery rate (90.12-102.00%) in milk sold on the open market, indicating that it has broad application possibilities in the food industry and biological medicine.
本文通过液相剥离法构建了一种新型的复合非酶过氧化氢（HO）传感器，由氧化铜、氧化亚铜和银纳米粒子掺杂少层石墨烯（CuO/Ag@FLG）组成。通过扫描电子显微镜（SEM）和 X 射线光电子能谱（XPS）对其表面形貌和组成进行了表征，并采用电化学方法研究了其 HO 传感性能，包括催化还原和定量检测。我们的传感器在 10 μM 至 100 mM 的极宽浓度范围内具有 174.5 μA mM cm 的高灵敏度（R = 0.9978），响应速度快（约 5 s），检测限低（S/N = 3）为 2.13 μM。该传感器在存在各种生物干扰物如多巴胺、抗坏血酸、尿酸、柠檬酸等时表现出出色的选择性。此外，构建的传感器在储存 1 个月后仍保持 95%的电流响应性，进一步表明其具有长期稳定性。最后但同样重要的是，它在市场上销售的牛奶中具有良好的回收率（90.12-102.00%），表明它在食品工业和生物医学领域具有广阔的应用前景。
