Instituto Patagónico para el Estudio de los Ecosistemas Continentales, Consejo Nacional de Investigaciones Científicas y Técnicas (IPEEC-CONICET), Puerto Madryn, U9120ACD, Argentina.
Instituto de Diversidad y Evolución Austral, Consejo Nacional de Investigaciones Científicas y Técnicas (IDEAus-CONICET), Puerto Madryn, U9120ACD, Argentina.
Syst Biol. 2023 Aug 7;72(4):739-752. doi: 10.1093/sysbio/syad019.
In this study we detangled the evolutionary history of the Patagonian lizard clade Liolaemus kingii, coupling dense geographic sampling and novel computational analytical approaches. We analyzed nuclear and mitochondrial data (restriction site-associated DNA sequencing and cytochrome b) to hypothesize and evaluate species limits, phylogenetic relationships, and demographic histories. We complemented these analyses with posterior predictive simulations to assess the fit of the genomic data to the multispecies coalescent model. We also employed a novel approach to time-calibrate a phylogenetic network. Our results show several instances of mito-nuclear discordance and consistent support for a reticulated history, supporting the view that the complex evolutionary history of the kingii clade is characterized by extensive gene flow and rapid diversification events. We discuss our findings in the contexts of the "gray zone" of speciation, phylogeographic patterns in the Patagonian region, and taxonomic outcomes. [Model adequacy; multispecies coalescent; multispecies network coalescent; phylogenomics; species delimitation.].
在这项研究中，我们结合密集的地理采样和新颖的计算分析方法，梳理了巴塔哥尼亚蜥蜴类群 Liolaemus kingii 的进化历史。我们分析了核和线粒体数据（限制性位点相关 DNA 测序和细胞色素 b），以假设和评估物种界限、系统发育关系和种群历史。我们通过后验预测模拟来补充这些分析，以评估基因组数据与多物种合并模型的拟合程度。我们还采用了一种新方法对系统发育网络进行时间校准。我们的结果显示了几种线粒体-核不符的情况，并一致支持网状进化历史，这表明 kingii 类群复杂的进化历史以广泛的基因流和快速多样化事件为特征。我们在“灰色地带”的物种形成、巴塔哥尼亚地区的系统地理格局和分类学结果等方面讨论了我们的发现。
