Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Mol Plant Microbe Interact. 2023 Jul;36(7):447-451. doi: 10.1094/MPMI-10-22-0210-A. Epub 2023 Aug 14.
The maize anthracnose stalk rot and leaf blight diseases caused by the fungal pathogen is emerging as an important threat to corn production worldwide. In this work, we provide an improved genome assembly of a strain (TZ-3) by using the PacBio Sequel II and Illumina high-throughput sequencing technologies. The genome of TZ-3 consists of 36 contigs with a length of 59.3 Mb. After correction and evaluation with the Illumina sequencing data and BUSCO, this genome showed a high assembly quality and integrity. Gene annotation of this genome predicted 11,911 protein-coding genes, among which 983 secreted protein-coding genes and 332 effector genes were predicted. Compared with previous genomes of strains, TZ-3 genome is superior in nearly all parameters. The genome assembly and annotation will enhance our knowledge of the genetic makeup of the pathogen and molecular mechanisms underlying its pathogenicity and will provide valuable insights into genome variation across different regions. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
玉米炭疽茎腐和叶斑病是由真菌病原体引起的,正在成为全球玉米生产的一个重要威胁。在这项工作中,我们使用 PacBio Sequel II 和 Illumina 高通量测序技术,对一个 菌株(TZ-3)进行了改进的基因组组装。TZ-3 的基因组由 36 个长度为 59.3Mb 的 contigs 组成。经过 Illumina 测序数据和 BUSCO 的校正和评估,这个基因组显示出了很高的组装质量和完整性。该基因组的基因注释预测了 11911 个编码蛋白的基因,其中预测了 983 个分泌蛋白编码基因和 332 个效应基因。与以前的 菌株基因组相比,TZ-3 基因组在几乎所有参数上都更优越。基因组的组装和注释将增强我们对病原体遗传组成和致病性分子机制的认识,并为不同地区的基因组变异提供有价值的见解。 [公式:见正文] 版权所有©2023 作者。这是一个在 CC BY-NC-ND 4.0 国际许可下发布的开放获取文章。