甲状腺乳头状癌中 DNA 甲基化与淋巴结转移的相关性。

Correlation of DNA methylation and lymph node metastasis in papillary thyroid carcinoma.

机构信息

Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Department of Head and Neck Surgery, Cancer Hospital of China Medical University, Shenyang, China.

出版信息

Head Neck. 2023 Jul;45(7):1654-1662. doi: 10.1002/hed.27377. Epub 2023 Apr 25.

DOI:10.1002/hed.27377

PMID:37097909

Abstract

BACKGROUND

Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer with a primarily good prognosis, and its 10-year survival rate is over 90%. However, PTC is prone to early lymph node metastasis.

METHODS

Thyroid cancer tissues from PTC patients with lymphatic metastasis and normal tissues were collected for DNA methylation analysis. Different methylation sites, different methylation regions, gene-enriched pathways, and protein-protein interactions (PPIs) were analyzed.

RESULTS

There were 1004 differentially methylated sites in the PTC group versus the control group; these involved 479 hypermethylated sites in 415 related genes, 525 hypomethylated sites in 482 related genes, 64 differentially methylated regions located in the CpG island region, 34 differentially methylated genes closely related to thyroid cancer, and 17 genes with differentially methylated genes in the DNA promoter region.

CONCLUSION

NDRG4 hypermethylation and FOXO3, ZEB2, and CDK6 hypomethylation were associated with PTC lymph node metastasis.

摘要

背景

甲状腺乳头状癌（PTC）是最常见的甲状腺癌类型，预后主要良好，其 10 年生存率超过 90%。然而，PTC 易发生早期淋巴结转移。

方法

收集 PTC 伴淋巴结转移患者和正常组织的甲状腺癌组织进行 DNA 甲基化分析。分析不同的甲基化位点、不同的甲基化区域、基因富集途径和蛋白质-蛋白质相互作用（PPIs）。

结果

PTC 组与对照组相比有 1004 个差异甲基化位点；涉及 415 个相关基因中的 479 个高甲基化位点、482 个相关基因中的 525 个低甲基化位点、64 个位于 CpG 岛区域的差异甲基化区域、34 个与甲状腺癌密切相关的差异甲基化基因和 17 个在 DNA 启动子区域具有差异甲基化基因的基因。