Inhibitory effect of 12-O-tetradecanoylphorbol 13-acetate on acid secretion by rat stomach in vivo.

The effect of 12-O-tetradecanoylphorbol 13-acetate (TPA) on acid secretion by rat stomach in vivo has been investigated to establish whether a previously determined inhibitory effect of TPA on aminopyrine accumulation by isolated parietal cells genuinely reflected a reduced acid secretion. Perfusion of 1 microM TPA through the lumen of the stomach had little effect on basal or carbachol-stimulated secretion, but inhibited secretion induced by histamine or pentagastrin. 4 alpha-Phorbol 12,13-didecanoate did not affect pentagastrin-stimulated secretion. It is concluded that TPA exerts a specific inhibitory effect on acid secretion in vivo, which parallels that found on aminopyrine accumulation in isolated parietal cells.