() plays an important role in intestinal regulation, while it is presently unclear whether live or pasteurized has different effects on the intestinal health. In the present study, live or pasteurized was administered to dextran sulfate sodium (DSS)-induced ulcerative colitis mice to investigate its influences on the host intestinal health, gut microbiota, and metabolomic phenotype. The results showed that pasteurized alleviated the symptoms of colitis in mice more efficiently by encouraging the proliferation of beneficial intestinal bacteria, increasing the production of short-chain fatty acids, and inhibiting intestinal inflammation. Moreover, pasteurized increased the abundance of and and thus affected the metabolism of lipid and lipid-like molecules associated with lysophosphatidylcholines (LysoPCs). Notably, prophylactic supplementation of pasteurized increased the relative abundance of the anti-inflammatory microbe , thereby activating intestinal sphingolipid metabolism to alleviate intestinal damage. In conclusion, pasteurized showed a more effective amelioration of DSS-induced colitis by repairing the dysbiosis of gut microbiota and intestinal metabolism compared to live , providing a potential strategy to explore the protective effect of on host intestinal health.