Li Lu, Sun Chenglong, Li Jiahui, Liu Jialin, Li Yunlong, Xie Qingji
Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China.
Mikrochim Acta. 2023 Apr 26;190(5):192. doi: 10.1007/s00604-023-05775-2.
AgBiS was hydrothermally synthesized, InO was synthesized by hydrothermal method and calcination, and the type-II InO/AgBiS heterojunction material of an optimized composition ratio was cast-coated on a fluorine-doped tin oxide (FTO) slice to fabricate an InO/AgBiS/FTO photoanode. The signal-attenuated photoelectrochemistry sandwich immunoassay of squamous cell carcinoma antigen (SCCA) was realized on this photoanode, on the basis of a bovine serum albumin/secondary antibody/CuO nanoparticles/nitrogen-doped porous carbon-ZnO bionanocomposite that can competitively absorb light and deplete the electron donor ascorbic acid as well as show the steric hindrance and p-n quenching effects. Under the optimized conditions (e.g., at a bias of 0 V vs. SCE), the photocurrent was linear with the common logarithm of SCCA concentration from 2.00 pg mL to 50.0 ng mL, with a limit of detection (LOD) of 0.62 pg mL (S/N = 3). The immunoassay of SCCA in human serum samples gave satisfactory recovery (92.0103%) and relative standard deviation (5.17.8%) results.
采用水热法合成了AgBiS，通过水热法和煅烧合成了InO，并将优化组成比的II型InO/AgBiS异质结材料浇铸涂覆在氟掺杂氧化锡（FTO）片上，制备了InO/AgBiS/FTO光阳极。基于牛血清白蛋白/二抗/CuO纳米颗粒/氮掺杂多孔碳-ZnO生物纳米复合材料，该光阳极实现了鳞状细胞癌抗原（SCCA）的信号衰减光电化学夹心免疫分析，该复合材料能够竞争性吸收光并耗尽电子供体抗坏血酸，同时表现出空间位阻和p-n猝灭效应。在优化条件下（例如，相对于饱和甘汞电极的偏压为0 V），光电流与SCCA浓度的常用对数在2.00 pg/mL至50.0 ng/mL范围内呈线性关系，检测限（LOD）为0.62 pg/mL（S/N = 3）。人血清样品中SCCA的免疫分析获得了令人满意的回收率（92.0103%）和相对标准偏差（5.17.8%）结果。
