（氨基氧基）乙酸对大鼠肝脏蛋白质合成的抑制作用。

Inhibition of protein synthesis by (aminooxy)acetate in rat liver.

作者信息

Girbes T, Alonso P

出版信息

Int J Biochem. 1986;18(6):537-42. doi: 10.1016/0020-711x(86)90165-5.

Abstract

(Aminooxy)acetate and D-cycloserine, two inhibitors of hepatic transamination reactions, inhibited also protein synthesis in isolated cells and postmitochondrial supernatants from rat liver. Both inhibitors acted in extracts only in concentrations higher than 1 mM. However, while D-cycloserine acted in isolated cells, as in extracts, (aminooxy)acetate inhibits protein synthesis in isolated cells by 50% of the control in the range 0.03-3 mM. NH+4 and H2O2, two by-products of (aminooxy)acetate degradation, inhibited protein synthesis in isolated liver cells, but at such a high concentration that the inhibition of protein synthesis carried out by (aminooxy)acetate cannot be explained by generation of these species. The results point out that the inhibitory action of (aminooxy)acetate on protein synthesis appears to require the integrity of the molecule.

摘要

（氨氧基）乙酸和D - 环丝氨酸这两种肝转氨反应抑制剂，也抑制大鼠肝脏分离细胞和线粒体后上清液中的蛋白质合成。两种抑制剂仅在浓度高于1 mM时才在提取物中起作用。然而，虽然D - 环丝氨酸在分离细胞中的作用与在提取物中一样，但（氨氧基）乙酸在0.03 - 3 mM范围内可使分离细胞中的蛋白质合成抑制50%。（氨氧基）乙酸降解的两种副产物NH₄⁺和H₂O₂可抑制分离的肝细胞中的蛋白质合成，但浓度过高，以至于（氨氧基）乙酸对蛋白质合成的抑制作用无法用这些物质的产生来解释。结果表明，（氨氧基）乙酸对蛋白质合成的抑制作用似乎需要分子的完整性。