胃肠道肿瘤中纤维母细胞活化蛋白过表达：系统评价与Meta分析方案

Fibroblast Activation Protein Overexpression in Gastrointestinal Tumors: Protocol for a Systematic Review and Meta-analysis.

作者信息

Sunami Yoshiaki, Ronellenfitsch Ulrich, Kleeff Jorg, Rebelo Artur

机构信息

Department of Visceral, Vascular and Endocrine Surgery, University Hospital Halle (Saale), Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany.

出版信息

JMIR Res Protoc. 2023 Apr 26;12:e45176. doi: 10.2196/45176.

DOI:10.2196/45176

PMID:37099374

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10173040/

Abstract

BACKGROUND

A hallmark of gastrointestinal cancer, especially pancreatic cancer, is the dense stromal tumor microenvironment in which cancer-associated fibroblasts (CAFs) represent the major stromal cell type. Preclinical studies have demonstrated that depletion of fibroblast activation protein (FAP)-positive CAFs results in increased survival.

OBJECTIVE

We present the protocol for a systematic review and meta-analysis that aim to assess the currently available evidence on the effect of FAP expression on survival and clinical characteristics in gastrointestinal cancers.

METHODS

The literature search and data analysis will be conducted in accordance with the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) 2020 statement. The databases PubMed/MEDLINE, Web of Science Core Collection, Cochrane Library, and ClinicalTrials.gov will be searched via their respective online search engines. A meta-analysis comparing patients with and without FAP overexpression with the following outcomes will be performed: postoperative survival (overall and median survival; 1-, 2-, 3-, and 5-year survival rates), histological differentiation (grading), local tumor invasion, lymph node metastases, and distant metastases. Odds ratios will be calculated for binary data, and weighted mean differences and relative SD differences will be determined for continuous data. The 95% CI, heterogeneity measures, and statistical significance will be reported for each outcome. The chi-square and Kruskal-Wallis tests will be used to evaluate statistical significance. A P value of <.05 will be considered statistically significant.

RESULTS

Database searches will commence in April 2023. The meta-analysis will be completed by December 2023.

CONCLUSIONS

In recent years, several publications on FAP overexpression in gastrointestinal tumors have been published. The only published meta-analysis on this topic dates to 2015. It included 15 studies on various solid tumors and only 8 studies focusing exclusively on gastrointestinal tumors. The expected results of the present analysis will provide new evidence on the prognostic value of FAP in gastrointestinal tumors and thereby support health care professionals and patients in their decision-making.

TRIAL REGISTRATION

PROSPERO CRD42022372194; https://tinyurl.com/352ae8b8.

INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): PRR1-10.2196/45176.

摘要

背景

胃肠道癌，尤其是胰腺癌的一个标志是致密的基质肿瘤微环境，其中癌症相关成纤维细胞（CAF）是主要的基质细胞类型。临床前研究表明，成纤维细胞活化蛋白（FAP）阳性的CAF减少会导致生存期延长。

目的

我们提出一项系统评价和荟萃分析方案，旨在评估目前关于FAP表达对胃肠道癌生存期和临床特征影响的现有证据。

方法

文献检索和数据分析将按照PRISMA（系统评价和荟萃分析首选报告项目）2020声明进行。将通过各自的在线搜索引擎检索PubMed/MEDLINE、科学网核心合集、Cochrane图书馆和ClinicalTrials.gov数据库。将对有和没有FAP过表达的患者进行荟萃分析，分析以下结果：术后生存期（总生存期和中位生存期；1年、2年、3年和5年生存率）、组织学分化（分级）、局部肿瘤侵犯、淋巴结转移和远处转移。将计算二元数据的比值比，并确定连续数据的加权平均差和相对标准差差异。将报告每个结果的95%置信区间、异质性度量和统计学显著性。将使用卡方检验和Kruskal-Wallis检验来评估统计学显著性。P值<0.05将被认为具有统计学显著性。