College of Life Science, Yangtze University, Jingzhou 434000, PR China.
College of Life Science, Yangtze University, Jingzhou 434000, PR China; Jingzhou Changxin Biotechnology Co., Ltd., Jingzhou 434000, PR China.
Acta Histochem. 2023 May;125(4):152030. doi: 10.1016/j.acthis.2023.152030. Epub 2023 Apr 24.
Superoxide dismutase (SOD) is an antioxidant enzyme with multiple metal cofactors that can specifically clear reactive oxygen species (ROS), which plays an important role in a variety of ultraviolet-induced lesions. Therefore, SOD has the anti-ultraviolet radiation effect. The objective of this study was to compare the differences in the anti-ultraviolet radiation effect of SOD with distinct metal cofactors: Cu/Zn-SOD and Mn-SOD. SOD was first purified using hydrophobic interaction chromatography and ion-exchange chromatography. Second, the Methylthiazolyldiphenyl-tetrazolium bromide method and cell senescence kits were used to study the protective effect of SOD on ultraviolet-induced cell damage. Finally, the protective effect of SOD on ultraviolet -induced skin damage was histopathologically evaluated, and the expression levels of malondialdehyde (MDA) and matrix metalloproteinases (MMPs) in tissues were detected. The results showed that Cu/Zn-SOD was superior to Mn-SOD in promoting cell proliferation, alleviating cell damage, protecting skin structure, and regulating the expression levels of MDA and MMPs, and it has no side effects. In conclusion, Cu/Zn-SOD had a better anti-ultraviolet radiation effect than Mn-SOD, and it can be used in anti-aging and anti-ultraviolet skin-care products.
超氧化物歧化酶（SOD）是一种具有多种金属辅基的抗氧化酶，可以特异性清除活性氧（ROS），在多种紫外线诱导损伤中发挥重要作用。因此，SOD 具有抗紫外线辐射的作用。本研究旨在比较具有不同金属辅基的 SOD（Cu/Zn-SOD 和 Mn-SOD）的抗紫外线辐射作用差异。首先，采用疏水相互作用色谱和离子交换色谱法对 SOD 进行纯化。其次，采用 Methylthiazolyldiphenyl-tetrazolium bromide 法和细胞衰老试剂盒研究 SOD 对紫外线诱导的细胞损伤的保护作用。最后，对 SOD 对紫外线诱导皮肤损伤的保护作用进行组织病理学评价，并检测组织中丙二醛（MDA）和基质金属蛋白酶（MMPs）的表达水平。结果表明，Cu/Zn-SOD 在促进细胞增殖、减轻细胞损伤、保护皮肤结构以及调节 MDA 和 MMPs 的表达水平方面优于 Mn-SOD，且无副作用。综上所述，Cu/Zn-SOD 的抗紫外线辐射作用优于 Mn-SOD，可用于抗衰老和防晒护肤品中。
