Department of Pharmaceutics, School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, 826 Zhangheng Road, Shanghai 201203, PR China.
Department of Pharmaceutics, School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, 826 Zhangheng Road, Shanghai 201203, PR China.
J Control Release. 2023 Jun;358:382-397. doi: 10.1016/j.jconrel.2023.04.035. Epub 2023 May 12.
Precisely reporting the inflammatory microenvironment, which can provide important basis for disease diagnosis and progression determination, is always challenging. In this work, we develop a targeting peptide-conjugated chemiluminescent reporter (OFF), which can be recognized by in-situ circulated neutrophils once injected, and then transported to the inflamed tissues characterized with overexpressed superoxide anion (O), assisted by the chemotaxis natural of neutrophils. Subsequently, the chemiluminescent probe can particularly respond to O to release the caged photons (ON) for visualizing inflammatory diseases such as subcutaneous tumor, colorectal cancer peritoneal metastasis (CCPM), ear swelling and kidney failure. The chemiluminescent probe provides a reliable tool to early detect inflammation and precisely excise micrometastatic lesions under optical guidance. This study provides a potential approach for improving the performance of luminophore in advanced bioimaging applications.
准确报告炎症微环境，可为疾病诊断和进展确定提供重要依据，但这始终具有挑战性。在这项工作中，我们开发了一种靶向肽偶联化学发光报告物（OFF），一旦注射，该报告物可被原位循环中的中性粒细胞识别，然后在中性粒细胞趋化作用的帮助下被运送到过表达超氧阴离子（O）的炎症组织中。随后，化学发光探针可以特别响应 O 释放被封闭的光子（ON），从而可视化皮下肿瘤、结直肠癌腹膜转移（CCPM）、耳部肿胀和肾衰竭等炎症性疾病。化学发光探针为早期检测炎症并在光学引导下精确切除微转移病变提供了一种可靠的工具。这项研究为提高发光体在高级生物成像应用中的性能提供了一种潜在的方法。
