Cadmium exposure induces a sex-dependent decline in left ventricular cardiac function.

AIMS

Cadmium exposure is a worldwide problem that has been linked to the development of cardiovascular disease. This study aimed to elucidate mechanistic details of chronic cadmium exposure on the structure and function of the heart.

MAIN METHODS

Male and female mice were exposed to cadmium chloride (CdCl) via drinking water for eight weeks. Serial echocardiography and blood pressure measurements were performed. Markers of hypertrophy and fibrosis were assessed, along with molecular targets of Ca-handling.

KEY FINDINGS

Males exhibited a significant reduction in left ventricular ejection fraction and fractional shortening with CdCl exposure, along with increased ventricular volume at end-systole, and decreased interventricular septal thickness at end-systole. Interestingly, no changes were detected in females. Experiments in isolated cardiomyocytes revealed that CdCl-induced contractile dysfunction was also present at the cellular level, showing decreased Ca transient and sarcomere shortening amplitude with CdCl exposure. Further mechanistic investigation uncovered a decrease in sarco/endoplasmic reticulum Ca-ATPase 2a (SERCA2a) protein expression and phosphorylated phospholamban levels in male hearts with CdCl exposure.

SIGNIFICANCE

The findings of our novel study provide important insight into how cadmium exposure may act as a sex-specific driver of cardiovascular disease, and further underscore the importance of reducing human exposure to cadmium.